Germination, senescence and pathogenic attack in soybean (Glycine max. L.): Identification of the cytosolic aconitase participating in the glyoxylate cycle

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Version: author
Serval ID
serval:BIB_0F4C02390F37
Type
Article: article from journal or magazin.
Collection
Publications
Title
Germination, senescence and pathogenic attack in soybean (Glycine max. L.): Identification of the cytosolic aconitase participating in the glyoxylate cycle
Journal
Plant Science
Author(s)
Cots J., Widmer F.
ISSN
0168-9452
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
149
Number
2
Pages
95-104
Language
english
Abstract
During our study of the glyoxylate cycle in soybean (Glycine max. L. var. Maple arrow), two mitochondrial and three cytosolic aconitase molecular species (EC 4.2.1.3) were detected, designated as M1, M2, C1, C2 and C3 isoforms, respectively, according to their intracellular locations and electrophoretic mobilities. Using the glyoxylate cycle marker enzymes isocitrate lyase (ICL, EC 4.1.3.1) and malate synthase (MS, EC 4.1.3.2), the activity of this pathway providing the essential link between P-oxidation and gluconeogenesis was confirmed during germination (cotyledons) and senescence (leaves). It was then established that, in both cases, the activity of the CI aconitase isoform developed concomitantly with the transcription and translation levels of the icl and ms genes. This strongly suggests that C1 aconitase is constitutive of the glyoxylate cycle. In addition, the same isoform was found to be active during pathogenic attack as well (hypocotyls). It might be assumed that in such a case the glyoxylate cycle is reinitiated as a part of a carbon reallocation system feeding on the diseased tissue cellular components.
Keywords
Glycine max. L., aconitase, germination, glyoxylate cycle, pathogenic attack, senescence, ETIOLATED PUMPKIN COTYLEDONS, MALATE SYNTHASE ACTIVITY, ISOCITRATE LYASE, ENZYME-ACTIVITIES, GENE-EXPRESSION, LEAF SENESCENCE, CUCUMBER COTYLEDONS, CANDIDA-TROPICALIS, BRASSICA-NAPUS, PEROXISOMES
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13/08/2015 8:54
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15/12/2019 8:08
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