Harvest site influences the growth properties of adipose derived stem cells.

Details

Serval ID
serval:BIB_0E77F6162153
Type
Article: article from journal or magazin.
Collection
Publications
Title
Harvest site influences the growth properties of adipose derived stem cells.
Journal
Cytotechnology
Author(s)
Engels P.E., Tremp M., Kingham P.J., di Summa P.G., Largo R.D., Schaefer D.J., Kalbermatten D.F.
ISSN
0920-9069 (Print)
ISSN-L
0920-9069
Publication state
Published
Issued date
2013
Volume
65
Number
3
Pages
437-445
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The therapeutic potential of adult stem cells may become a relevant option in clinical care in the future. In hand and plastic surgery, cell therapy might be used to enhance nerve regeneration and help surgeons and clinicians to repair debilitating nerve injuries. Adipose-derived stem cells (ASCs) are found in abundant quantities and can be harvested with a low morbidity. In order to define the optimal fat harvest location and detect any potential differences in ASC proliferation properties, we compared biopsies from different anatomical sites (inguinal, flank, pericardiac, omentum, neck) in Sprague-Dawley rats. ASCs were expanded from each biopsy and a proliferation assay using different mitogenic factors, basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF) was performed. Our results show that when compared with the pericardiac region, cells isolated from the inguinal, flank, omental and neck regions grow significantly better in growth medium alone. bFGF significantly enhanced the growth rate of ASCs isolated from all regions except the omentum. PDGF had minimal effect on ASC proliferation rate but increases the growth of ASCs from the neck region. Analysis of all the data suggests that ASCs from the neck region may be the ideal stem cell sources for tissue engineering approaches for the regeneration of nervous tissue.
Pubmed
Web of science
Create date
10/10/2016 7:59
Last modification date
02/06/2020 5:26
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