Nanoparticle conjugation enhances the immunomodulatory effects of intranasally delivered CpG in house dust mite-allergic mice.

Details

Serval ID
serval:BIB_0E381269A57B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Nanoparticle conjugation enhances the immunomodulatory effects of intranasally delivered CpG in house dust mite-allergic mice.
Journal
Scientific Reports
Author(s)
Ballester M., Jeanbart L., de Titta A., Nembrini C., Marsland B.J., Hubbell J.A., Swartz M.A.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
5
Pages
14274
Language
english
Notes
Publication types: Journal ArticlePublication Status: epublish
Abstract
An emerging strategy in preventing and treating airway allergy consists of modulating the immune response induced against allergens in the lungs. CpG oligodeoxynucleotides have been investigated in airway allergy studies, but even if promising, efficacy requires further substantiation. We investigated the effect of pulmonary delivery of nanoparticle (NP)-conjugated CpG on lung immunity and found that NP-CpG led to enhanced recruitment of activated dendritic cells and to Th1 immunity compared to free CpG. We then evaluated if pulmonary delivery of NP-CpG could prevent and treat house dust mite-induced allergy by modulating immunity directly in lungs. When CpG was administered as immunomodulatory therapy prior to allergen sensitization, we found that NP-CpG significantly reduced eosinophilia, IgE levels, mucus production and Th2 cytokines, while free CpG had only a moderate effect on these parameters. In a therapeutic setting where CpG was administered after allergen sensitization, we found that although both free CpG and NP-CpG reduced eosinophilia and IgE levels to the same extent, NP conjugation of CpG significantly enhanced reduction of Th2 cytokines in lungs of allergic mice. Taken together, these data highlight benefits of NP conjugation and the relevance of NP-CpG as allergen-free therapy to modulate lung immunity and treat airway allergy.
Keywords
Adjuvants, Immunologic/administration & dosage, Adjuvants, Immunologic/therapeutic use, Administration, Inhalation, Animals, Cytokines/biosynthesis, Cytokines/drug effects, Dendritic Cells/drug effects, Dendritic Cells/immunology, Hypersensitivity/drug therapy, Hypersensitivity/immunology, Mice, Nanoparticles/administration & dosage, Nanoparticles/therapeutic use, Oligodeoxyribonucleotides/administration & dosage, Oligodeoxyribonucleotides/therapeutic use, Pyroglyphidae/immunology, T-Lymphocytes, Helper-Inducer/drug effects, T-Lymphocytes, Helper-Inducer/immunology
Pubmed
Web of science
Open Access
Yes
Create date
13/10/2015 17:52
Last modification date
20/08/2019 12:35
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