Differentiation of rat striatal embryonic stem cells in vitro: monolayer culture vs. three-dimensional coculture with differentiated brain cells.

Details

Serval ID
serval:BIB_0DE30CEF8FFE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differentiation of rat striatal embryonic stem cells in vitro: monolayer culture vs. three-dimensional coculture with differentiated brain cells.
Journal
Journal of Neuroscience Research
Author(s)
Pardo B., Honegger P.
ISSN
0360-4012 (Print)
ISSN-L
0360-4012
Publication state
Published
Issued date
2000
Volume
59
Number
4
Pages
504-512
Language
english
Abstract
Several groups have demonstrated the existence of self-renewing stem cells in embryonic and adult mouse brain. In vitro, these cells proliferate in response to epidermal growth factor, forming clusters of nestin-positive cells that may be dissociated and subcultured repetitively. Here we show that, in stem cell clusters derived from rat embryonic striatum, cell proliferation decreased with increasing number of passages and in response to elevated concentrations of potassium (30 mM KCl). In monolayer culture, the appearance of microtubule-associated protein type-5-immunoreactive (MAP-5(+)) cells (presumptive neurons) in response to basic fibroblast growth factor (bFGF) was reduced at low cell density and with increasing number of passages. In the presence of bFGF, elevated potassium caused a more differentiated neuronal phenotype, characterized by an increased proportion of MAP-5(+) cells, extensive neuritic branching, and higher specific activity of glutamic acid decarboxylase. Dissociated stem cells were able to invade cultured brain cell aggregates containing different proportions of neurons and glial cells, whereas they required the presence of a considerable proportion of glial cells in the host cultures to become neurofilament H-positive. The latter observation supports the view that astrocyte-derived factors influence early differentiation of the neuronal cell lineage.
Keywords
Animals, Cell Differentiation/drug effects, Cell Differentiation/physiology, Cells, Cultured, Coculture Techniques/methods, Corpus Striatum/cytology, Corpus Striatum/drug effects, Epidermal Growth Factor/pharmacology, Fibroblast Growth Factor 2/pharmacology, Mice, Microtubule-Associated Proteins/drug effects, Microtubule-Associated Proteins/metabolism, Neuroglia/drug effects, Neuroglia/physiology, Neurons/drug effects, Neurons/physiology, Potassium Chloride/pharmacology, Rats, Stem Cells/drug effects, Stem Cells/physiology
Pubmed
Web of science
Create date
24/01/2008 13:11
Last modification date
20/08/2019 12:34
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