Reduction-Sensitive Protein Nanogels Enhance Uptake of Model and Tumor Lysate Antigens In Vitro by Mouse- and Human-Derived Dendritic Cells.

Details

Serval ID
serval:BIB_0D82E169FC14
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Reduction-Sensitive Protein Nanogels Enhance Uptake of Model and Tumor Lysate Antigens In Vitro by Mouse- and Human-Derived Dendritic Cells.
Journal
ACS applied bio materials
Author(s)
Berti C., Boarino A., Graciotti M., Bader LPE, Kandalaft L.E., Klok H.A.
ISSN
2576-6422 (Electronic)
ISSN-L
2576-6422
Publication state
Published
Issued date
20/12/2021
Peer-reviewed
Oui
Volume
4
Number
12
Pages
8291-8300
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Peptides and proteins represent an emerging class of powerful therapeutics. Peptide and protein nanogels are attractive carriers for the transport and delivery of biologically active peptides and proteins because they allow essentially quantitative encapsulation of these biologics. One interesting field of use of peptide and protein nanogels is the transport of antigens and adjuvants in cancer immunotherapy. This study demonstrates the use of reduction-sensitive protein nanogels for the delivery of ovalbumin and oxidized tumor lysate-based antigens to mouse and human-donor-derived dendritic cells. Challenging mouse-derived and human dendritic cells with reduction-sensitive ovalbumin nanogels was found to significantly enhance antigen uptake as compared to the use of the corresponding free protein antigen. The experiments with mouse-derived dendritic cells further showed that the administration of ovalbumin in the form of reduction-sensitive nanogels enhanced dendritic cell maturation as well as the presentation of the SIINFEKL epitope as compared to experiments that use free ovalbumin. In addition to ovalbumin as a model antigen, the feasibility of reduction-sensitive nanogels was also demonstrated for the delivery of oxidized, whole tumor lysate-based cancer antigens. In experiments with dendritic cells harvested from human donors, dendritic cell uptake of the oxidized tumor lysate antigen was significantly enhanced in experiments that used oxidized tumor lysate nanogels as compared to the free antigen.
Keywords
antigen delivery, cancer vaccines, nanogels, proteins, tumor lysate
Pubmed
Open Access
Yes
Create date
17/01/2022 12:10
Last modification date
28/01/2022 7:33
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