Drosophila melanogaster dHCF Interacts with both PcG and TrxG Epigenetic Regulators.

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Serval ID
serval:BIB_0B43BEBE64F0
Type
Article: article from journal or magazin.
Collection
Publications
Title
Drosophila melanogaster dHCF Interacts with both PcG and TrxG Epigenetic Regulators.
Journal
PLoS One
Author(s)
Rodriguez-Jato S., Busturia A., Herr W.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2011
Volume
6
Number
12
Pages
e27479
Language
english
Abstract
Repression and activation of gene transcription involves multiprotein complexes that modify chromatin structure. The integration of these complexes at regulatory sites can be assisted by co-factors that link them to DNA-bound transcriptional regulators. In humans, one such co-factor is the herpes simplex virus host-cell factor 1 (HCF-1), which is implicated in both activation and repression of transcription. We show here that disruption of the gene encoding the Drosophila melanogaster homolog of HCF-1, dHCF, leads to a pleiotropic phenotype involving lethality, sterility, small size, apoptosis, and morphological defects. In Drosophila, repressed and activated transcriptional states of cell fate-determining genes are maintained throughout development by Polycomb Group (PcG) and Trithorax Group (TrxG) genes, respectively. dHCF mutant flies display morphological phenotypes typical of TrxG mutants and dHCF interacts genetically with both PcG and TrxG genes. Thus, dHCF inactivation enhances the mutant phenotypes of the Pc PcG as well as brm and mor TrxG genes, suggesting that dHCF possesses Enhancer of TrxG and PcG (ETP) properties. Additionally, dHCF interacts with the previously established ETP gene skd. These pleiotropic phenotypes are consistent with broad roles for dHCF in both activation and repression of transcription during fly development.
Pubmed
Web of science
Open Access
Yes
Create date
16/01/2012 15:42
Last modification date
20/08/2019 12:33
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