Fasting induces metabolic switches and spatial redistributions of lipid processing and neuronal interactions in tanycytes.

Details

Serval ID
serval:BIB_0B3E856627D3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Fasting induces metabolic switches and spatial redistributions of lipid processing and neuronal interactions in tanycytes.
Journal
Nature communications
Author(s)
Brunner M., Lopez-Rodriguez D., Estrada-Meza J., Dali R., Rohrbach A., Deglise T., Messina A., Thorens B., Santoni F., Langlet F.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
04/08/2024
Peer-reviewed
Oui
Volume
15
Number
1
Pages
6604
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
The ependyma lining the third ventricle (3V) in the mediobasal hypothalamus plays a crucial role in energy balance and glucose homeostasis. It is characterized by a high functional heterogeneity and plasticity, but the underlying molecular mechanisms governing its features are not fully understood. Here, 5481 hypothalamic ependymocytes were cataloged using FACS-assisted scRNAseq from fed, 12h-fasted, and 24h-fasted adult male mice. With standard clustering analysis, typical ependymal cells and β2-tanycytes appear sharply defined, but other subpopulations, β1- and α-tanycytes, display fuzzy boundaries with few or no specific markers. Pseudospatial approaches, based on the 3V neuroanatomical distribution, enable the identification of specific versus shared tanycyte markers and subgroup-specific versus general tanycyte functions. We show that fasting dynamically shifts gene expression patterns along the 3V, leading to a spatial redistribution of cell type-specific responses. Altogether, we show that changes in energy status induce metabolic and functional switches in tanycyte subpopulations, providing insights into molecular and functional diversity and plasticity within the tanycyte population.
Keywords
Animals, Ependymoglial Cells/metabolism, Male, Fasting/metabolism, Mice, Neurons/metabolism, Lipid Metabolism, Ependyma/metabolism, Ependyma/cytology, Hypothalamus/metabolism, Hypothalamus/cytology, Mice, Inbred C57BL, Energy Metabolism, Third Ventricle/metabolism, Glucose/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
09/08/2024 14:03
Last modification date
31/10/2024 7:13
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