Inhibition of a slow synaptic response by a metabotropic glutamate receptor antagonist in hippocampal CA3 pyramidal cells.

Details

Serval ID
serval:BIB_0B3E31FF1FC1
Type
Article: article from journal or magazin.
Collection
Publications
Title
Inhibition of a slow synaptic response by a metabotropic glutamate receptor antagonist in hippocampal CA3 pyramidal cells.
Journal
Proceedings of the Royal Society B Biological Sciences
Author(s)
Gerber U., Lüthi A., Gähwiler B.H.
ISSN
0962-8452 (Print)
ISSN-L
0962-8452
Publication state
Published
Issued date
1993
Peer-reviewed
Oui
Volume
254
Number
1340
Pages
169-172
Language
english
Abstract
The effects of a novel antagonist of metabotropic glutamate receptors were investigated in CA3 pyramidal cells in hippocampal slice cultures of the rat. Earlier experiments showed that selective activation of metabotropic glutamate receptors with low concentrations of an agonist, 1S, 3R-1-amino-cyclopentane-1,3-dicarboxylic acid (ACPD), induced an inward current associated with a decrease in membrane conductance and inhibition of the slow calcium-dependent potassium current. These responses were strongly and reversibly reduced by the antagonist, (RS)-alpha-methyl-4-carboxyphenylglycine (MCPG, 0.5-1 mM). In the presence of antagonists of ionotropic glutamate receptors, stimulation of the afferent mossy fibres evoked postsynaptic responses in CA3 pyramidal cells which paralleled those observed with exogenously applied metabotropic glutamate receptor agonists, i.e. a slow inward current and a reduction of calcium-dependent potassium current. Both responses were greatly reduced by bath-applied MCPG (1 mM). These results show that MCPG acts as an effective antagonist at metabotropic glutamate receptors coupled to potassium conductances in the hippocampus. Furthermore, they confirm that glutamate release from presynaptic terminals can modulate postsynaptic properties by activation of metabotropic glutamate receptors.
Keywords
6-Cyano-7-nitroquinoxaline-2,3-dione, Animals, Benzoates/pharmacology, Bicuculline/pharmacology, Cycloleucine/analogs & derivatives, Cycloleucine/pharmacology, Evoked Potentials/drug effects, Glycine/analogs & derivatives, Glycine/pharmacology, Hippocampus/physiology, Nerve Fibers/drug effects, Nerve Fibers/physiology, Neurotoxins/pharmacology, Pyramidal Cells/drug effects, Pyramidal Cells/physiology, Quinoxalines/pharmacology, Rats, Receptors, Glutamate/metabolism, Synapses/drug effects, Synapses/physiology
Pubmed
Web of science
Create date
15/08/2012 8:24
Last modification date
20/08/2019 12:33
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