Multinucleated giant cells with an osteoclast phenotype derived from caprine peripheral blood mononuclear cells.

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Serval ID
serval:BIB_0B2278F0D3B3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Production externe
Title
Multinucleated giant cells with an osteoclast phenotype derived from caprine peripheral blood mononuclear cells.
Journal
Veterinary journal
Author(s)
Pisoni G., D'Amelio P., Sassi F., Manarolla G., Scaccabarozzi L., Locatelli C., Mazzocchi N., Baruscotti M., DiFrancesco D., Moroni P.
ISSN
1532-2971 (Electronic)
ISSN-L
1090-0233
Publication state
Published
Issued date
09/2011
Peer-reviewed
Oui
Volume
189
Number
3
Pages
361-363
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Formation of multinucleated giant cells (MGCs) by macrophage fusion is a typical cytopathic effect of lentiviral replication in caprine monocytes and MGC formation from cultured caprine peripheral blood mononuclear cells (PBMCs) has been considered to be diagnostic for small ruminant lentivirus (SRLV) infection. In this study, formation of MGCs was observed after 7-14 days when PBMCs were cultured from healthy goats free from SRLV infection. These MGCs expressed tartrate-resistant acid phosphatase, calcitonin receptor, integrin αVβ3, cathepsin K and matrix metalloproteinase 9 and were able to resorb bone in vitro in the absence of RANKL and macrophage colony stimulating factor, consistent with an osteoclast phenotype.
Keywords
Acid Phosphatase/genetics, Acid Phosphatase/metabolism, Animals, Bone and Bones/metabolism, Cells, Cultured, Gene Expression Regulation, Giant Cells/pathology, Goats, Isoenzymes/genetics, Isoenzymes/metabolism, Leukocytes, Mononuclear/metabolism, Macrophage Colony-Stimulating Factor/genetics, Macrophage Colony-Stimulating Factor/metabolism, Membrane Proteins/genetics, Membrane Proteins/metabolism, Monocytes/metabolism, Osteoclasts/cytology, Osteoclasts/metabolism, Osteoclasts/pathology, Peptide Hydrolases/genetics, Peptide Hydrolases/metabolism, Phenotype, RANK Ligand/genetics, RANK Ligand/metabolism, Reverse Transcriptase Polymerase Chain Reaction/veterinary, Tartrate-Resistant Acid Phosphatase
DOI
10.1016/j.tvjl.2010.07.016
Pubmed
20822938
Web of science
000296407100027
Create date
16/01/2020 15:24
Last modification date
17/01/2020 7:26
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