Endogenous opioid peptides in parasympathetic, sympathetic and sensory nerves in the guinea-pig heart.

Details

Serval ID
serval:BIB_0B183D4F4EBA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Endogenous opioid peptides in parasympathetic, sympathetic and sensory nerves in the guinea-pig heart.
Journal
Cell and Tissue Research
Author(s)
Steele P.A., Aromataris E.C., Riederer B.M.
ISSN
0302-766X (Print)
ISSN-L
0302-766X
Publication state
Published
Issued date
1996
Volume
284
Number
2
Pages
331-339
Language
english
Abstract
Research has suggested that exogenous opioid substances can have direct effects on cardiac muscle or influence neurotransmitter release via presynaptic modulation of neuronal inputs to the heart. In the present study, multiple-labelling immunohistochemistry was employed to determine the distribution of endogenous opioid peptides within the guinea-pig heart. Approximately 40% of cardiac ganglion cells contained immunoreactivity for dynorphin A (1-8), dynorphin A (1-17) and dynorphin B whilst 20% displayed leu-enkephalin immunoreactivity. Different populations of opioid-containing ganglion cells were identified according to the co-existence of opioid immunoreactivity with immunoreactivity for somatostatin and neuropeptide Y. Immunoreactivity for prodynorphin-derived peptides was observed in many sympathetic axons in the heart and was also observed, though to a lesser extent, in sensory axons. Leu-enkephalin immunoreactivity was observed in occasional sympathetic and sensory axons. No immunoreactivity was observed for met-enkephalin-arg-gly-leu or for beta-endorphin. These results demonstrate that prodynorphin-derived peptides are present in parasympathetic, sympathetic and sensory nerves within the heart, but suggest that only the prodynorphin gene is expressed in guinea-pig cardiac nerves. This study has shown that endogenous opioid peptides are well placed to regulate cardiac function via both autonomic and sensory pathways.
Keywords
Adrenergic Fibers/chemistry, Animals, Antibody Specificity, Axons/chemistry, Dynorphins/analysis, Dynorphins/immunology, Endorphins/analysis, Endorphins/immunology, Enkephalin, Leucine/analysis, Enkephalin, Leucine/immunology, Enkephalin, Methionine/analogs & derivatives, Enkephalin, Methionine/analysis, Enkephalins/analysis, Enkephalins/immunology, Female, Ganglia, Autonomic/chemistry, Guinea Pigs, Heart/innervation, Immunohistochemistry, Male, Neurons, Afferent/chemistry, Opioid Peptides/analysis, Parasympathetic Fibers, Postganglionic/chemistry, Protein Precursors/analysis, Protein Precursors/immunology, beta-Endorphin/analysis, beta-Endorphin/immunology
Pubmed
Web of science
Create date
24/01/2008 15:35
Last modification date
20/08/2019 13:32
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