Genomic organization and subchromosomal in situ localization of the murine granzyme F, a serine protease expressed in CD8+ T cells

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Serval ID
serval:BIB_0AA9FED4DF1C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Genomic organization and subchromosomal in situ localization of the murine granzyme F, a serine protease expressed in CD8+ T cells
Journal
Journal of Immunology
Author(s)
Jenne  D. E., Zimmer  M., Garcia-Sanz  J. A., Tschopp  J., Lichter  P.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
08/1991
Volume
147
Number
3
Pages
1045-52
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 1
Abstract
Granzyme F belongs to a closely related family of seven murine serine proteases stored in cytoplasmic granules of lymphoid cell populations. In contrast to the murine granzymes A to E and G, granzyme F is exclusively expressed in the CD4-CD8+ subset of peripheral T cells. To characterize the genomic sequences responsible for its highly restricted expression, we isolated a cosmid clone and sequenced a 7.5-kb genomic fragment that contains the promoter region and all five exons of the murine granzyme F gene. A TATA box sequence is located at position -25 relative to the transcription initiation site, which was determined by RNase protection. The genomic organization of granzyme F is similar to that of granzyme B and granzyme C, leukocyte elastase, cathepsin G, rat mast cell protease II, and complement factor D (adipsin). By the use of two fluorochromes for simultaneous high resolution in situ hybridization, the granzyme F gene was localized in close proximity distally from the TCR alpha-chain locus on mouse chromosome 14.
Keywords
Amino Acid Sequence Animals Base Sequence Exons/genetics Granzymes Killer Cells, Natural/*metabolism Mice/*genetics Molecular Sequence Data Nucleic Acid Hybridization Promoter Regions (Genetics)/genetics Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Nucleic Acid Serine Endopeptidases/*genetics TATA Box/genetics
Pubmed
Web of science
Create date
24/01/2008 16:19
Last modification date
20/08/2019 13:32
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