Bacterial community structure of a pesticide-contaminated site and assessment of changes induced in community structure during bioremediation.

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It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
Serval ID
serval:BIB_0A14F850ECDE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Bacterial community structure of a pesticide-contaminated site and assessment of changes induced in community structure during bioremediation.
Journal
Fems Microbiology Ecology
Author(s)
Paul D., Pandey G., Meier C., van der Meer J.R., Jain R.K.
ISSN
0168-6496 (Print)
ISSN-L
0168-6496
Publication state
Published
Issued date
2006
Volume
57
Number
1
Pages
116-127
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The introduction of culture-independent molecular screening techniques, especially based on 16S rRNA gene sequences, has allowed microbiologists to examine a facet of microbial diversity not necessarily reflected by the results of culturing studies. The bacterial community structure was studied for a pesticide-contaminated site that was subsequently remediated using an efficient degradative strain Arthrobacter protophormiae RKJ100. The efficiency of the bioremediation process was assessed by monitoring the depletion of the pollutant, and the effect of addition of an exogenous strain on the existing soil community structure was determined using molecular techniques. The 16S rRNA gene pool amplified from the soil metagenome was cloned and restriction fragment length polymorphism studies revealed 46 different phylotypes on the basis of similar banding patterns. Sequencing of representative clones of each phylotype showed that the community structure of the pesticide-contaminated soil was mainly constituted by Proteobacteria and Actinomycetes. Terminal restriction fragment length polymorphism analysis showed only nonsignificant changes in community structure during the process of bioremediation. Immobilized cells of strain RKJ100 enhanced pollutant degradation but seemed to have no detectable effects on the existing bacterial community structure.
Keywords
Arthrobacter/metabolism, Biodegradation, Environmental, Ecosystem, Gene Library, India, Methyl Parathion/metabolism, Molecular Sequence Data, Nitrophenols/metabolism, Parathion/metabolism, Polymorphism, Restriction Fragment Length, Population Dynamics, Principal Component Analysis/methods, RNA, Ribosomal, 16S/genetics, Soil Microbiology
Pubmed
Web of science
Open Access
Yes
Create date
21/01/2008 14:36
Last modification date
25/09/2019 7:08
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