The identification of pathogenic yeast strains by electrophoretic analysis of their chromosomes

Details

Serval ID
serval:BIB_09EA5408D651
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The identification of pathogenic yeast strains by electrophoretic analysis of their chromosomes
Journal
Journal of Medical Microbiology
Author(s)
Monod  M., Porchet  S., Baudraz-Rosselet  F., Frenk  E.
ISSN
0022-2615 (Print)
Publication state
Published
Issued date
06/1990
Volume
32
Number
2
Pages
123-9
Notes
Journal Article --- Old month value: Jun
Abstract
Epidemiological studies require characterisation of pathogenic yeasts at and below the species level. The chromosomes of 130 strains of four pathogenic species of the genus Candida, isolated from clinical material, were separated by pulsed field electrophoresis with the clamped homogeneous electric field (CHEF) technique. Each species was characterised by a distinct electrophoretic karyotype (EK). Furthermore, smaller variations of the EK amongst strains belonging to the same species appeared to offer a useful means of strain differentiation. A karyotyping system is proposed for C. albicans. The EKs were assigned to a code of four numbers which designated the number of bands that could be resolved in each of four sets of chromosomes. Morphotypes of the colonies of C. albicans on malt agar plates, which did not correlate with the EK, could provide a complementary means of strain characterisation in epidemiological studies.
Keywords
Candida/*classification/genetics/growth & development Candida albicans/*classification/genetics/growth & development Chromosomes, Fungal/*analysis DNA, Fungal/*analysis Electrophoresis, Agar Gel Humans Karyotyping
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 16:47
Last modification date
20/08/2019 12:32
Usage data