Acylated monogalactosyl diacylglycerol: prevalence in the plant kingdom and identification of an enzyme catalyzing galactolipid head group acylation in Arabidopsis thaliana.

Details

Serval ID
serval:BIB_09EA35FE3A73
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Acylated monogalactosyl diacylglycerol: prevalence in the plant kingdom and identification of an enzyme catalyzing galactolipid head group acylation in Arabidopsis thaliana.
Journal
The Plant journal
Author(s)
Nilsson A.K., Johansson O.N., Fahlberg P., Kommuri M., Töpel M., Bodin L.J., Sikora P., Modarres M., Ekengren S., Nguyen C.T., Farmer E.E., Olsson O., Ellerström M., Andersson M.X.
ISSN
1365-313X (Electronic)
ISSN-L
0960-7412
Publication state
Published
Issued date
12/2015
Peer-reviewed
Oui
Volume
84
Number
6
Pages
1152-1166
Language
english
Abstract
The lipid phase of the thylakoid membrane is mainly composed of the galactolipids mono- and digalactosyl diacylglycerol (MGDG and DGDG, respectively). It has been known since the late 1960s that MGDG can be acylated with a third fatty acid to the galactose head group (acyl-MGDG) in plant leaf homogenates. In certain brassicaceous plants like Arabidopsis thaliana, the acyl-MGDG frequently incorporates oxidized fatty acids in the form of the jasmonic acid precursor 12-oxo-phytodienoic acid (OPDA). In the present study we further investigated the distribution of acylated and OPDA-containing galactolipids in the plant kingdom. While acyl-MGDG was found to be ubiquitous in green tissue of plants ranging from non-vascular plants to angiosperms, OPDA-containing galactolipids were only present in plants from a few genera. A candidate protein responsible for the acyl transfer was identified in Avena sativa (oat) leaf tissue using biochemical fractionation and proteomics. Knockout of the orthologous gene in A. thaliana resulted in an almost total elimination of the ability to form both non-oxidized and OPDA-containing acyl-MGDG. In addition, heterologous expression of the A. thaliana gene in E. coli demonstrated that the protein catalyzed acylation of MGDG. We thus demonstrate that a phylogenetically conserved enzyme is responsible for the accumulation of acyl-MGDG in A. thaliana. The activity of this enzyme in vivo is strongly enhanced by freezing damage and the hypersensitive response.
Keywords
Acyltransferases/genetics, Acyltransferases/metabolism, Arabidopsis/enzymology, Arabidopsis/genetics, Arabidopsis/metabolism, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Galactolipids/chemistry, Galactolipids/metabolism, Gene Deletion, Gene Expression Regulation, Enzymologic/physiology, Gene Expression Regulation, Plant/physiology, Phylogeny, Tobacco/metabolism, Arabidopsis thaliana, Avena sativa, acylated MGDG, galactolipids, hypersensitive response, thylakoid membrane, wounding
Pubmed
Web of science
Open Access
Yes
Create date
01/11/2016 19:55
Last modification date
01/01/2021 6:24
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