Oxidative potential for fine/ultrafine particles in occupational situations

Details

Serval ID
serval:BIB_09AAF77B2322
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Abstract (Abstract): shot summary in a article that contain essentials elements presented during a scientific conference, lecture or from a poster.
Collection
Publications
Institution
Title
Oxidative potential for fine/ultrafine particles in occupational situations
Title of the conference
4th International Conference on Nanotechnology - Occupational and Environmental Health, 26 - 29 August 2009, Helsinki Congress Paasitorni, Helsinki, Finland
Author(s)
Sauvain Jean-Jacques, Deslarzes Simon, Storti Ferdinand, Riediker Michael
Publisher
Finnish Institute of Occupational Health
Address
Helsinki
Publication state
Published
Issued date
2009
Pages
75
Language
english
Notes
SAPHIRID:81062
Abstract
Introduction : The redox properties of fine/ultrafine particles as well as nanoparticles (NP) are suggested to be important to explain their biological activity and could constitute a novel and promising metric for hazard evaluation. The acellular in vitro dithiothreitol (DTT) assay allows measuring this property.
Objectives : (1) to evaluate sampling requirements for fine/ultrafine particle allowing measurement of their oxidative potential (2) to apply the methodology to occupational situations where particle from combustion sources are generated.
Material and method : Sampling parameters (type of filters and loaded amount) and storage duration affecting the DTT measurements were evaluated. Based on these results, a methodological approach was defined and applied in two occupational situations where diesel and other combustion particles are present (toll station in a tunnel and mechanical yard for bus reparation).
Results : Teflon filters loaded with diesel particles were found more suitable for the DTT assay, due to their better chemical inertness compared to quartz filters: after storage durations larger than 150 hours, an increased reactivity toward DTT was observed only with quartz filters. Reactivity was linearly correlated to the loaded mass until about 1000 μg/filter. Different redox reactivities were determined in both working places, with the mechanical yard presenting a higher DTT consumption rate.
Discussion and conclusions : These results demonstrate the feasibility of this method to determine the oxidative potential of fine/ultrafine particles in occupational situations. We propose to include this approach for hazard assessment of work places with exposure to manufactured and other NP.
Keywords
Aerosols , Particulate Matter , Oxidative Stress , Mechanics , Biological Markers , Occupational Exposure , Chemistry, Analytical
Create date
27/01/2010 12:20
Last modification date
20/08/2019 12:31
Usage data