Adipose-derived stem cells enhance peripheral nerve regeneration.

Details

Serval ID
serval:BIB_0933C6CB51E9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Adipose-derived stem cells enhance peripheral nerve regeneration.
Journal
Journal of Plastic, Reconstructive and Aesthetic Surgery
Author(s)
di Summa P.G., Kingham P.J., Raffoul W., Wiberg M., Terenghi G., Kalbermatten D.F.
ISSN
1878-0539 (Electronic)
ISSN-L
1748-6815
Publication state
Published
Issued date
2010
Volume
63
Number
9
Pages
1544-1552
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Traumatic injuries resulting in peripheral nerve lesions often require a graft to bridge the gap. Although autologous nerve auto-graft is still the first-choice strategy in reconstructions, it has the severe disadvantage of the sacrifice of a functional nerve. Cell transplantation in a bioartificial conduit is an alternative strategy to create a favourable environment for nerve regeneration. We decided to test new fibrin nerve conduits seeded with various cell types (primary Schwann cells and adult stem cells differentiated to a Schwann cell-like phenotype) for repair of sciatic nerve injury. Two weeks after implantation, the conduits were removed and examined by immunohistochemistry for axonal regeneration (evaluated by PGP 9.5 expression) and Schwann cell presence (detected by S100 expression). The results show a significant increase in axonal regeneration in the group of fibrin seeded with Schwann cells compared with the empty fibrin conduit. Differentiated adipose-derived stem cells also enhanced regeneration distance in a similar manner to differentiated bone marrow mesenchymal stem cells. These observations suggest that adipose-derived stem cells may provide an effective cell population, without the limitations of the donor-site morbidity associated with isolation of Schwann cells, and could be a clinically translatable route towards new methods to enhance peripheral nerve repair.
Keywords
Adipocytes/transplantation, Analysis of Variance, Animals, Biocompatible Materials/pharmacology, Cell Differentiation/physiology, Fibrin Tissue Adhesive/pharmacology, Guided Tissue Regeneration/methods, Immunohistochemistry, Microsurgery, Nerve Regeneration/physiology, Rats, Rats, Sprague-Dawley, Schwann Cells/cytology, Schwann Cells/transplantation, Sciatic Nerve/cytology, Sciatic Nerve/injuries, Stem Cell Transplantation/methods
Pubmed
Web of science
Create date
16/12/2009 16:17
Last modification date
20/08/2019 12:31
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