Expression of Cre recombinase in pigment cells

Details

Serval ID
serval:BIB_0927AF1EC235
Type
Article: article from journal or magazin.
Collection
Publications
Title
Expression of Cre recombinase in pigment cells
Journal
Pigment Cell Research
Author(s)
Guyonneau  L., Rossier  A., Richard  C., Hummler  E., Beermann  F.
ISSN
0893-5785 (Print)
Publication state
Published
Issued date
08/2002
Volume
15
Number
4
Pages
305-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Abstract
Conditional gene targeting using the Cre/loxP system enables specific deletion of a gene in a tissue of interest. For application of Cre-mediated recombination in pigment cells, Cre expression has to be targeted to pigment cells in transgenic mice. So far, no pigment cell-specific Cre transgenic line has been reported and we present and discuss our first results on use of Cre recombinase in pigment cells. A construct was generated where Cre recombinase is controlled by the promoter of the mouse dopachrome tautomerase (Dct) gene. The construct was functionally tested in vitro and introduced into mice. Following breeding to two reporter mouse strains, we detected Cre recombinase activity in telencephalon, melanoblasts, and retinal pigment epithelium (RPE). Our data demonstrate the feasibility of pigment cell-specific Cre/loxP-mediated recombination.
Keywords
Animals Cells, Cultured Codon, Terminator/genetics Female Gene Targeting/*methods Green Fluorescent Proteins Integrases/*diagnostic use/*genetics Intramolecular Oxidoreductases/genetics Luminescent Proteins/genetics Male Melanins/biosynthesis/*genetics Melanocytes/cytology/*enzymology Mice Mice, Transgenic Pigment Epithelium of Eye/cytology/*enzymology Promoter Regions (Genetics)/genetics Recombinant Fusion Proteins/genetics Viral Proteins/*diagnostic use/*genetics
Pubmed
Web of science
Create date
24/01/2008 13:42
Last modification date
03/03/2018 13:31
Usage data