Electron-microscopic demonstration of terminal and internal initiation sites for cDNA synthesis on vitellogenin mRNA.

Details

Serval ID
serval:BIB_0923C115C8AC
Type
Article: article from journal or magazin.
Collection
Publications
Title
Electron-microscopic demonstration of terminal and internal initiation sites for cDNA synthesis on vitellogenin mRNA.
Journal
European Journal of Biochemistry / FEBS
Author(s)
Wahli W., Wyler T., Weber R., Ryffel G.U.
ISSN
0014-2956[print], 0014-2956[linking]
Publication state
Published
Issued date
05/1978
Volume
86
Number
1
Pages
225-234
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
cDNA synthesized on purified vitellogenin mRNA from Xenopus liver was hybridized to the template in formamide/urea at 22 degrees C to avoid degradation of the RNA. The hybrids formed were visualized by spreading for electron microscopy. Contour length measurements proved that most of the RNA molecules in the hybrids were still intact showing the expected molecular weight of 2.3 x 10(6). The hybridized cDNA corresponded on the average to 12% of the RNA length. In about 80% of the molecules the cDNA was located at one end. Since cDNA synthesis was primed by oligo(dT), the terminal duplex region marks the 3' end of the vitellogenin mRNA molecule. Internal duplex regions were mainly located at a specific position starting about 2800 nucleotides from the 3' end. Since the cDNA hybridizing at the internal position could specifically be synthesized on a vitellogenin RNA fragment isolated on poly(U)-Sepharose as an oligo(A)-containing RNA, we conclude that cDNA synthesis is not only initiated by the poly(A) of the 3' end, but also by a specific internal sequence.
Keywords
Animals, DNA/biosynthesis, Female, Kinetics, Lipoproteins/biosynthesis, Macromolecular Substances, Microscopy, Electron, Molecular Weight, Nucleic Acid Conformation, Nucleic Acid Hybridization, RNA, Messenger/metabolism, Vitellogenins/biosynthesis, Xenopus
Pubmed
Web of science
Create date
24/01/2008 16:04
Last modification date
20/08/2019 12:31
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