Tumor cells suppress cytokine-induced nitric-oxide (NO) production in cerebral endothelial cells

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State: Public
Version: Final published version
License: All rights reserved
Serval ID
serval:BIB_08A749FEBB67
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Tumor cells suppress cytokine-induced nitric-oxide (NO) production in cerebral endothelial cells
Journal
International Journal of Cancer
Author(s)
Murata J., Corradin S. B., Janzer R. C., Juillerat-Jeanneret L.
ISSN
0020-7136 (Print)
Publication state
Published
Issued date
1994
Volume
59
Number
5
Pages
699-705
Language
english
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Abstract
Nitric oxide (NO) produced by endothelial cells (EC) has been shown to exert cytotoxic activity on tumor cells. In order to analyze events involved in brain metastasis, the modulation of NO production in rat-brain-derived EC was investigated. NO release was increased by tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukin-1 beta, lipopolysaccharide or forskolin in EC219 cells, a rat-brain-microvessel-derived EC line. Dexamethasone decreased NO release by cytokine-activated EC219 cells. Tumor cells (DHD/K12/PROb, a rat colon-carcinoma cell line) were highly adherent to EC219 cells, and adhesion was not modified by TNF-alpha plus IFN-gamma, or by dexamethasone. Addition of tumor cells or tumor-cell-conditioned medium significantly inhibited NO release induced by any of the stimuli examined, but only if added during the initial phase of endothelial-cell activation. Tumor-derived suppression of NO release was also observed in primary cultures of cerebral EC. NO synthase (NOS) activity in cytosol extracts of the cerebral EC line was Ca(2+)-independent and required both NADPH and tetrahydrobiopterin. NOS activity was increased by TNF-alpha and IFN-gamma, and significantly reduced by tumor-cell-conditioned medium. These results suggest that rat colon-carcinoma cells may have developed a protective mechanism involving the release of (a) soluble factor(s) which inhibit(s) NO production by cerebral EC
Keywords
Amino Acid Oxidoreductases/metabolism/Animals/Biopterin/analogs & derivatives/pharmacology/Brain/blood supply/Cell Adhesion/Cell Line/Colonic Neoplasms/Culture Media,Conditioned/Cytokines/Dexamethasone/Endothelium,Vascular/Forskolin/Interferon Type II/Interleukin-1/Lipopolysaccharides/Nadp/Nitric Oxide/biosynthesis/Nitric Oxide Synthase/Rats/Tumor Cells,Cultured/Tumor Necrosis Factor-alpha
Pubmed
Web of science
Create date
29/01/2008 19:34
Last modification date
14/07/2020 7:08
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