Mini invasive skeletal muscle biopsy technique with a tri-axial end cut needle.

Details

Ressource 1Request a copy Sous embargo indéterminé.
State: Public
Version: Final published version
License: All rights reserved
Serval ID
serval:BIB_08825D4BF422
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Mini invasive skeletal muscle biopsy technique with a tri-axial end cut needle.
Journal
European Review For Medical and Pharmacological Sciences
Author(s)
Invernizzi M., Rizzi M., Carda S., Cisari C., Molinari C., Renò F.
ISSN
2284-0729 (Electronic)
ISSN-L
1128-3602
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
19
Number
13
Pages
2446-2451
Language
english
Abstract
OBJECTIVE: Skeletal Muscle Biopsy is a minor surgical procedure for the diagnosis of different neuromuscular pathological conditions and has recently gained popularity also in the research field of age-related muscular modifications and sarcopenia. Few studies focused on the application of mini-invasive muscular biopsy in both normal and pathological conditions. The aim of our study was to describe a mini invasive ultrasound-guided skeletal muscular biopsy technique in complete spinal cord injured (SCI) patients and healthy controls with a tri-axial end-cut needle.
PATIENTS AND METHODS: Skeletal muscle biopsies were collected from 6 chronic SCI patients and 3 healthy controls vastus lateralis muscle with a tri-axial end cut needle (Biopince© - Angiotech). Muscle samples were stained for ATPase to determine fibers composition, moreover, gene expression of cyclooxygenase-1 (COX-1) and prostaglandin E2 receptor has been analyzed by Real Time RT-PCR.
RESULTS: All the procedures were perfomed easily without failures and complications. Control tissue was macroscopically thicker than SCI one. Control specimen displayed an equal distribution of type I and type II fibers, while SCI sample displayed a prevalence of type II fibers SCI specimen displayed a significant reduction in COX-1 gene expression. This mini-invasive approach was easy, accurate and with low complication rate in performing skeletal muscle biopsy in both SCI patients and controls.
CONCLUSIONS: This technique could be useful in conditions in which the overall quantity of specimen required is small like for molecular biology analysis. For histological diagnostic purposes and/or conditions in which the original tissue is already pathologically modified, this technique should be integrated with more invasive techniques.
Pubmed
Web of science
Create date
28/08/2015 18:45
Last modification date
20/08/2019 13:30
Usage data