Site-directed introduction of disulfide groups on antibodies for highly sensitive immunosensors

Details

Serval ID
serval:BIB_07B01ED1FC7B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Site-directed introduction of disulfide groups on antibodies for highly sensitive immunosensors
Journal
Analytical and Bioanalytical Chemistry
Author(s)
Acero Sánchez Josep L., Fragoso Alex, Joda Hamdi, Suárez Guillaume, McNeil Calum J., O'Sullivan Ciara K.
ISSN
1618-2642 (Print)
1618-2650 (Electronic)
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
408
Number
19
Pages
5337-5346
Language
english
Abstract
The interface between the sample and the transducer surface is critical to the performance of a biosensor. In this work, we compared different strategies for covalent self-assembly of antibodies onto bare gold substrates by introducing disulfide groups into the immunoglobulin structure, which acted as anchor molecules able to chemisorb spontaneously onto clean gold surfaces. The disulfide moieties were chemically introduced to the antibody via the primary amines, carboxylic acids, and carbohydrates present in its structure. The site-directed modification via the carbohydrate chains exhibited the best performance in terms of analyte response using a model system for the detection of the stroke marker neuron-specific enolase. SPR measurements clearly showed the potential for creating biologically active densely packed self-assembled monolayers (SAMs) in a one-step protocol compared to both mixed SAMs of alkanethiol compounds and commercial immobilization layers. The ability of the carbohydrate strategy to construct an electrochemical immunosensor was investigated using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) transduction
Keywords
Biosensing Techniques/methods , Electrochemical Techniques/methods , Proteins/chemistry ,
Pubmed
Web of science
Create date
15/11/2016 13:42
Last modification date
20/08/2019 12:30
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