SH3 interactome conserves general function over specific form.

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Version: Final published version
Serval ID
serval:BIB_0758D1FD524C
Type
Article: article from journal or magazin.
Collection
Publications
Title
SH3 interactome conserves general function over specific form.
Journal
Molecular Systems Biology
Author(s)
Xin X., Gfeller D., Cheng J., Tonikian R., Sun L., Guo A., Lopez L., Pavlenco A., Akintobi A., Zhang Y., Rual J.F., Currell B., Seshagiri S., Hao T., Yang X., Shen Y.A., Salehi-Ashtiani K., Li J., Cheng A.T., Bouamalay D., Lugari A., Hill D.E., Grimes M.L., Drubin D.G., Grant B.D., Vidal M., Boone C., Sidhu S.S., Bader G.D.
ISSN
1744-4292 (Electronic)
ISSN-L
1744-4292
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
9
Pages
652
Language
english
Abstract
Src homology 3 (SH3) domains bind peptides to mediate protein-protein interactions that assemble and regulate dynamic biological processes. We surveyed the repertoire of SH3 binding specificity using peptide phage display in a metazoan, the worm Caenorhabditis elegans, and discovered that it structurally mirrors that of the budding yeast Saccharomyces cerevisiae. We then mapped the worm SH3 interactome using stringent yeast two-hybrid and compared it with the equivalent map for yeast. We found that the worm SH3 interactome resembles the analogous yeast network because it is significantly enriched for proteins with roles in endocytosis. Nevertheless, orthologous SH3 domain-mediated interactions are highly rewired. Our results suggest a model of network evolution where general function of the SH3 domain network is conserved over its specific form.

Keywords
Amino Acid Sequence, Animals, Caenorhabditis elegans/genetics, Caenorhabditis elegans/metabolism, Caenorhabditis elegans Proteins/genetics, Caenorhabditis elegans Proteins/metabolism, Conserved Sequence, Endocytosis/genetics, Evolution, Molecular, Molecular Sequence Data, Protein Interaction Mapping, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, Structural Homology, Protein, Two-Hybrid System Techniques, src Homology Domains/genetics
Pubmed
Web of science
Open Access
Yes
Create date
15/12/2014 13:20
Last modification date
20/08/2019 12:29
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