Tea4p links microtubule plus ends with the formin for3p in the establishment of cell polarity.

Details

Serval ID
serval:BIB_07215D83CEB3
Type
Article: article from journal or magazin.
Collection
Publications
Title
Tea4p links microtubule plus ends with the formin for3p in the establishment of cell polarity.
Journal
Developmental Cell
Author(s)
Martin S.G., McDonald W.H., Yates J.R., Chang F.
ISSN
1534-5807 (Print)
ISSN-L
1534-5807
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
8
Number
4
Pages
479-491
Language
english
Abstract
Microtubules regulate actin-based processes such as cell migration and cytokinesis, but molecular mechanisms are not understood. In the fission yeast Schizosaccharomyces pombe, microtubule plus ends regulate cell polarity in part by transporting the kelch repeat protein tea1p to cell ends. Here, we identify tea4p, a SH3 domain protein that binds directly to tea1p. Like tea1p, tea4p localizes to growing microtubule plus ends and to cortical sites at cell ends, and it is necessary for the establishment of bipolar growth. Tea4p binds directly to and recruits the formin for3p, which nucleates actin cable assembly. During "new end take off" (NETO), formation of a protein complex that includes tea1p, tea4p, and for3p is necessary and sufficient for the establishment of cell polarity and localized actin assembly at new cell ends. Our results suggest a molecular mechanism for how microtubule plus ends regulate the spatial distribution of actin assembly.
Keywords
Actins/metabolism, Amino Acid Sequence, Cell Cycle Proteins/genetics, Cell Cycle Proteins/metabolism, Cell Polarity, Cytoskeleton/metabolism, Microtubule-Associated Proteins/genetics, Microtubule-Associated Proteins/metabolism, Microtubules/metabolism, Molecular Sequence Data, Multiprotein Complexes, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism, Schizosaccharomyces/cytology, Schizosaccharomyces/genetics, Schizosaccharomyces pombe Proteins/genetics, Schizosaccharomyces pombe Proteins/metabolism, Sequence Alignment, Two-Hybrid System Techniques, src Homology Domains
Pubmed
Web of science
Open Access
Yes
Create date
18/03/2008 11:32
Last modification date
20/08/2019 12:29
Usage data