The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein

Details

Serval ID
serval:BIB_0710A46A718C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein
Journal
Journal of Bacteriology
Author(s)
Telenti  A., Southworth  M., Alcaide  F., Daugelat  S., Jacobs, W. R., Jr. , Perler  F. B.
ISSN
0021-9193 (Print)
Publication state
Published
Issued date
10/1997
Volume
179
Number
20
Pages
6378-82
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Oct
Abstract
The 198-amino-acid in-frame insertion in the gyrA gene of Mycobacterium xenopi is the smallest known naturally occurring active protein splicing element (intein). Comparison with other mycobacterial gyrA inteins suggests that the M. xenopi intein underwent a complex series of events including (i) removal of 222 amino acids that encompass most of the central intein domain, and (ii) addition of a linker of unrelated residues. This naturally occurring genetic rearrangement is a representative characteristic of the taxon. The deletion process removes the conserved motifs involved in homing endonuclease activity. The linker insertion represents a structural requirement, as its mutation resulted in failure to splice. The M. xenopi GyrA intein thus provides a paradigm for a minimal protein splicing element.
Keywords
Amino Acid Sequence Bacterial Proteins/chemistry/*genetics/metabolism Dithiothreitol/pharmacology Molecular Sequence Data Mycobacterium xenopi/chemistry/*genetics/metabolism *Protein Splicing Sequence Analysis Temperature
Pubmed
Web of science
Create date
25/01/2008 14:45
Last modification date
20/08/2019 12:29
Usage data