Two-photon 3-D mapping of ex vivo human skin endogenous fluorescence species based on fluorescence emission spectra.

Details

Serval ID
serval:BIB_06874070331E
Type
Article: article from journal or magazin.
Collection
Publications
Title
Two-photon 3-D mapping of ex vivo human skin endogenous fluorescence species based on fluorescence emission spectra.
Journal
Journal of Biomedical Optics
Author(s)
Laiho L.H., Pelet S., Hancewicz T.M., Kaplan P.D., So P.T.C.
ISSN
1083-3668 (Print)
ISSN-L
1083-3668
Publication state
Published
Issued date
2005
Volume
10
Number
2
Pages
024016
Language
english
Abstract
Spectral resolved tissue imaging has a broad range of biomedical applications such as the minimally invasive diagnosis of diseases and the study of wound healing and tissue engineering processes. Two-photon microscopy imaging of endogenous fluorescence has been shown to be a powerful method for the quantification of tissue structure and biochemistry. While two-photon excited autofluorescence is observed ubiquitously, the identities and distributions of endogenous fluorophores have not been completely characterized in most tissues. We develop an image-guided spectral analysis method to analyze the distribution of fluorophores in human skin from 3-D resolved two-photon images. We identify five factors that contribute to most of the luminescence signals from human skin. Luminescence species identified include tryptophan, NAD(P)H, melanin, and elastin, which are autofluorescent, and collagen that contributes to a second harmonic signal.
Keywords
Equipment Design, Humans, Imaging, Three-Dimensional, Luminescence, Microscopy, Fluorescence, Multiphoton/instrumentation, Models, Theoretical, Skin/anatomy & histology, Skin Physiological Phenomena
Pubmed
Web of science
Create date
25/09/2012 14:26
Last modification date
20/08/2019 12:28
Usage data