Pancreas preservation fluid microbial contamination is associated with poor islet isolation outcomes - a multi-centre cohort study

Details

Serval ID
serval:BIB_060FEC04BF3D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Pancreas preservation fluid microbial contamination is associated with poor islet isolation outcomes - a multi-centre cohort study
Journal
Transpl Int
Author(s)
Meier R. P. H., Andrey D. O., Sun P., Niclauss N., Bedat B., Demuylder-Mischler S., Borot S., Benhamou P. Y., Wojtusciszyn A., Buron F., Pernin N., Muller Y. D., Bosco D., van Delden C., Berney T.
ISSN
1432-2277 (Electronic)
ISSN-L
0934-0874
Publication state
Published
Issued date
08/2018
Volume
31
Number
8
Pages
917-929
Language
english
Notes
Meier, Raphael P H
Andrey, Diego O
Sun, Pamela
Niclauss, Nadja
Bedat, Benoit
Demuylder-Mischler, Sandrine
Borot, Sophie
Benhamou, Pierre-Yves
Wojtusciszyn, Anne
Buron, Fanny
Pernin, Nadine
Muller, Yannick D
Bosco, Domenico
van Delden, Christian
Berney, Thierry
eng
Multicenter Study
England
Transpl Int. 2018 Aug;31(8):917-929. doi: 10.1111/tri.13159. Epub 2018 Apr 16.
Abstract
The microbiological safety of islet preparations is paramount. Preservation medium contamination is frequent, and its impact on islet yield and function remains unclear. Microbiological samples collected during islet isolations from 2006 to 2016 were analyzed and correlated to isolation and allo- and autotransplantation outcomes. Microbial contamination of preservation medium was found in 64.4% of processed donor pancreases (291/452). We identified 464 microorganisms including Staphylococcus (253/464, 54.5%), Streptococcus (31/464, 6.7%), and Candida species (25/464, 5.4%). Microbial contamination was associated with longer warm and cold ischemia times and lower numbers of postpurification islet equivalents, purity, transplant rate, and stimulation index (all P < 0.05). Six percent of the preparations accepted for transplantation showed microbial contamination after isolation (12/200); 9 of 12 were Candida species. Six patients were transplanted with a sample with late microbial growth discovered after the infusion. Insulin independence rate was not affected. This risk of transplanting a contaminated islets preparation was reduced by half following the implementation of an additional sampling after 24 h of islet culture. Pancreas preservation fluid microbial contamination is associated with lower transplant rate and poorer in vitro function, but not with changes in graft survival. Culture medium testing 1 day after isolation reduces the risk of incidental transplantation with contaminated islets.
Keywords
Adolescent, Adult, Aged, Child, Cohort Studies, Drug Contamination/*statistics & numerical data, Female, Humans, Islets of Langerhans/microbiology, Islets of Langerhans Transplantation/*statistics & numerical data, Male, Middle Aged, *Organ Preservation Solutions, Young Adult, *islet isolation, *islet transplantation, *microbial contamination, *type 1 diabetes mellitus
Pubmed
Create date
14/06/2021 9:59
Last modification date
18/09/2021 6:38
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