Identification of a novel composite transposable element, Tn5280, carrying chlorobenzene dioxygenase genes of Pseudomonas sp. strain P51.

Details

Serval ID
serval:BIB_05EDE2A18F10
Type
Article: article from journal or magazin.
Collection
Publications
Title
Identification of a novel composite transposable element, Tn5280, carrying chlorobenzene dioxygenase genes of Pseudomonas sp. strain P51.
Journal
Journal of Bacteriology
Author(s)
van der Meer J.R., Zehnder A.J., de Vos W.M.
ISSN
0021-9193 (Print)
ISSN-L
0021-9193
Publication state
Published
Issued date
1991
Peer-reviewed
Oui
Volume
173
Number
22
Pages
7077-7083
Language
english
Abstract
Analysis of one of the regions of catabolic plasmid pP51 which encode chlorobenzene metabolism of Pseudomonas sp. strain P51 revealed that the tcbA and tcbB genes for chlorobenzene dioxygenase and dehydrogenase are located on a transposable element, Tn5280. Tn5280 showed the features of a composite bacterial transposon with iso-insertion elements (IS1066 and IS1067) at each end of the transposon oriented in an inverted position. When a 12-kb HindIII fragment of pP51 containing Tn5280 was cloned in the suicide donor plasmid pSUP202, marked with a kanamycin resistance gene, and introduced into Pseudomonas putida donor plasmid pSUP202, marked with a kanamycin resistance gene, and introduced into Pseudomonas putida KT2442, Tn5280 was found to transpose into the genome at random and in single copy. The insertion elements IS1066 and IS1067 differed in a single base apir located in the inner inverted repeat and were found to be highly homologous to a class of repetitive elements of Bradyrhizobium japonicum and distantly related to IS630 of Shigella sonnei. The presence of the catabolic genes tcbA and tcbB on Tn5280 suggests a mechanism by which gene clusters can be mobilized as gene cassettes and joined with others to form novel catabolic pathways.
Keywords
Amino Acid Sequence, Base Sequence, Conjugation, Genetic, DNA Transposable Elements, Dioxygenases, Drug Resistance, Microbial/genetics, Escherichia coli/genetics, Genes, Bacterial, Molecular Sequence Data, Open Reading Frames, Oxidoreductases/genetics, Oxygenases/genetics, Plasmids, Protein Conformation, Pseudomonas/enzymology, Pseudomonas/genetics, Repetitive Sequences, Nucleic Acid, Restriction Mapping, Rhizobiaceae/enzymology, Rhizobiaceae/genetics, Sequence Homology, Nucleic Acid, Software
Pubmed
Web of science
Create date
21/01/2008 13:36
Last modification date
20/08/2019 12:28
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