Hsp27 upregulation and phosphorylation is required for injured sensory and motor neuron survival
Details
Serval ID
serval:BIB_05DAC476DACF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Hsp27 upregulation and phosphorylation is required for injured sensory and motor neuron survival
Journal
Neuron
ISSN
0896-6273 (Print)
Publication state
Published
Issued date
09/2002
Volume
36
Number
1
Pages
45-56
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep 26
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep 26
Abstract
Peripheral nerve transection results in the rapid death by apoptosis of neonatal but not adult sensory and motor neurons. We show that this is due to induction and phosphorylation in all adult axotomized neurons of the small heat shock protein Hsp27 and the failure of such induction in most neonatal neurons. In vivo delivery of human Hsp27 but not a nonphosphorylatable mutant prevents neonatal rat motor neurons from nerve injury-induced death, while knockdown in vitro and in vivo of Hsp27 in adult injured sensory neurons results in apoptosis. Hsp27's neuroprotective action is downstream of cytochrome c release from mitochondria and upstream of caspase-3 activation. Transcriptional and posttranslational regulation of Hsp27 is necessary for sensory and motor neuron survival following peripheral nerve injury.
Keywords
Aging/metabolism
Animals
Animals, Newborn
Anterior Horn Cells/cytology/*metabolism
Apoptosis/physiology
Caspase 3
Caspases/metabolism
Cell Survival/*physiology
Cells, Cultured
Cytochrome c Group/metabolism
Ganglia, Spinal/cytology/growth & development/*metabolism
*Heat-Shock Proteins
Humans
Immunohistochemistry
Neoplasm Proteins/antagonists & inhibitors/genetics/*metabolism
Neurons, Afferent/cytology/*metabolism
Peripheral Nerves/growth & development/*injuries/metabolism
Phosphorylation
Rats
Rats, Sprague-Dawley
Recombinant Fusion Proteins/diagnostic use/genetics
Up-Regulation/*physiology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:45
Last modification date
20/08/2019 13:27