Frontline Science: Leishmania mexicana amastigotes can replicate within neutrophils.

Details

Serval ID
serval:BIB_059313F5EEE1
Type
Article: article from journal or magazin.
Collection
Publications
Title
Frontline Science: Leishmania mexicana amastigotes can replicate within neutrophils.
Journal
Journal of Leukocyte Biology
Author(s)
Hurrell B.P., Beaumann M., Heyde S., Regli I.B., Müller A.J., Tacchini-Cottier F.
ISSN
1938-3673 (Electronic)
ISSN-L
0741-5400
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
102
Number
5
Pages
1187-1198
Language
english
Abstract
Cutaneous leishmaniasis is a neglected tropical disease, causing a spectrum of clinical manifestations varying from self-healing to unhealing lesions that may be very difficult to treat. Emerging evidence points to a detrimental role for neutrophils during the first hours following infection with many distinct Leishmania species (spp.) at a time when the parasite is in its nonreplicative promastigote form. Neutrophils have also been detected at later stages of infection in unhealing chronic cutaneous lesions. However, the interactions between these cells and the replicative intracellular amastigote form of the parasite have been poorly studied. Here, we show that Leishmaniamexicana amastigotes are efficiently internalized by neutrophils and that this process has only a low impact on neutrophil activation and apoptosis. In neutrophils, the amastigotes were found in acidified vesicles. Furthermore, within cutaneous unhealing lesions, heavily infected neutrophils were found with up to 6 parasites per cell. To investigate if the amastigotes could replicate within neutrophils, we generated photoconvertible fluorescent parasites. With the use of flow cytometry imaging and time-lapse microscopy, we could demonstrate that a subset of parasites replicated within neutrophils. Overall, our data reveal a novel role for neutrophils that can act as a niche for parasite replication during the chronic phase of infection, thereby contributing to disease pathology.

Keywords
Animals, Cell Division, Female, Flow Cytometry, Fluorescent Dyes/metabolism, Genes, Reporter, Host-Parasite Interactions/immunology, Leishmania mexicana/growth & development, Leishmania mexicana/pathogenicity, Leishmania mexicana/ultrastructure, Leishmaniasis, Cutaneous/parasitology, Leishmaniasis, Cutaneous/pathology, Life Cycle Stages/genetics, Mice, Mice, Inbred C57BL, Neutrophils/parasitology, Neutrophils/ultrastructure, Organisms, Genetically Modified/growth & development, Phagocytosis, Photochemical Processes, Time-Lapse Imaging, granulocytes, photoconvertible parasite, promastigotes, replication
Pubmed
Web of science
Create date
13/11/2017 18:15
Last modification date
20/08/2019 12:27
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