Gene expression profile in human late radiation enteritis obtained by high-density cDNA array hybridization.
Details
Serval ID
serval:BIB_057E912DFE73
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Gene expression profile in human late radiation enteritis obtained by high-density cDNA array hybridization.
Journal
Radiation Research
ISSN
0033-7587 (Print)
ISSN-L
0033-7587
Publication state
Published
Issued date
2004
Peer-reviewed
Oui
Volume
161
Number
3
Pages
299-311
Language
english
Notes
Publication types: Clinical Trial ; Controlled Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't ; Validation StudiesPublication Status: ppublish
Abstract
Late radiation enteritis is a sequela of radiation therapy to the abdomen. The pathogenic process is poorly understood at the molecular level. cDNA array analysis was used to provide new insights into the pathogenesis of this disorder. Gene profiles of six samples of fibrotic bowel tissue from patients with radiation enteritis and six healthy bowel tissue samples from patients without radiation enteritis were compared using membrane-based arrays containing 1314 cDNAs. Results were confirmed with real-time RT-PCR and Western blot analysis. Array analysis identified many differentially expressed genes involved in fibrosis, stress response, inflammation, cell adhesion, intracellular and nuclear signaling, and metabolic pathways. Increased expression of genes coding for proteins involved in the composition and remodeling of the extracellular matrix, along with altered expression of genes involved in cell- to-cell and cell-to-matrix interactions, were observed mainly in radiation enteritis samples. Stress, inflammatory responses, and antioxidant metabolism were altered in radiation enteritis as were genes coding for recruitment of lymphocytes and macrophages. The Rho/HSP27 (HSPB1)/zyxin pathway, involved in tissue contraction and myofibroblast transdifferentiation, was also altered in radiation enteritis, suggesting that this pathway could be related to the fibrogenic process. Our results provide a global and integrated view of the alteration of gene expression associated with radiation enteritis. They suggest that radiation enteritis is a dynamic process involving constant remodeling of each structural component of the intestinal tissue, i.e. the mucosa, the mesenchyme, and blood vessels. Functional studies will be necessary to validate the present results.
Keywords
Adult, Aged, Enteritis/etiology, Enteritis/genetics, Female, Gene Expression Profiling/methods, Gene Expression Regulation/radiation effects, Humans, Ileum/pathology, Ileum/radiation effects, Male, Middle Aged, Neoplasms/radiotherapy, Oligonucleotide Array Sequence Analysis/methods, Radiation Genetics/methods, Radiation Injuries/etiology, Radiation Injuries/genetics, Radiotherapy/adverse effects, Time Factors
Pubmed
Web of science
Create date
01/12/2014 18:54
Last modification date
20/08/2019 13:27