Diffusion-weighted MRS of acetate in the rat brain.

Details

Serval ID
serval:BIB_055C63573E2D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Diffusion-weighted MRS of acetate in the rat brain.
Journal
NMR in biomedicine
Author(s)
Dehghani M., Kunz N., Lanz B., Yoshihara HAI, Gruetter R.
ISSN
1099-1492 (Electronic)
ISSN-L
0952-3480
Publication state
Published
Issued date
10/2017
Peer-reviewed
Oui
Volume
30
Number
10
Pages
1
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Acetate has been proposed as an astrocyte-specific energy substrate for metabolic studies in the brain. The determination of the relative contribution of the intracellular and extracellular compartments to the acetate signal using diffusion-weighted magnetic resonance spectroscopy can provide an insight into the cellular environment and distribution volume of acetate in the brain. In the present study, localized <sup>1</sup> H nuclear magnetic resonance (NMR) spectroscopy employing a diffusion-weighted stimulated echo acquisition mode (STEAM) sequence at an ultra-high magnetic field (14.1 T) was used to investigate the diffusivity characteristics of acetate and N-acetylaspartate (NAA) in the rat brain in vivo during prolonged acetate infusion. The persistence of the acetate resonance in <sup>1</sup> H spectra acquired at very large diffusion weighting indicated restricted diffusion of acetate and was attributed to intracellular spaces. However, the significantly greater diffusion of acetate relative to NAA suggests that a substantial fraction of acetate is located in the extracellular space of the brain. Assuming an even distribution for acetate in intracellular and extracellular spaces, the diffusion properties of acetate yielded a smaller volume of distribution for acetate relative to water and glucose in the rat brain.
Keywords
Acetates/metabolism, Animals, Aspartic Acid/analogs & derivatives, Aspartic Acid/metabolism, Brain/metabolism, Diffusion Magnetic Resonance Imaging, Male, Metabolome, Monte Carlo Method, Probability, Proton Magnetic Resonance Spectroscopy, Rats, Sprague-Dawley, acetate, diffusion, magnetic resonance spectroscopy, metabolites, rat brain, volume of distribution
Pubmed
Web of science
Create date
07/09/2017 8:06
Last modification date
20/08/2019 12:27
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