Aggregating Brain Cell Cultures as a Model to Study Ischaemia-induced Neurodegeneration.

Details

Serval ID
serval:BIB_0530308D59DF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Aggregating Brain Cell Cultures as a Model to Study Ischaemia-induced Neurodegeneration.
Journal
Toxicology in Vitro
Author(s)
Pardo B., Honegger P.
ISSN
0887-2333 (Print)
ISSN-L
0887-2333
Publication state
Published
Issued date
1999
Volume
13
Number
4-5
Pages
543-547
Language
english
Abstract
Rotation-mediated aggregating brain cell cultures at two different maturational stages (DIV 11 and DIV 20) were subjected for 1 or 2 hours to ischaemic conditions by transient immobilization (arrest of media circulation). During recovery, cell damage was evaluated by measuring changes in cell type-specific enzyme activities and total protein content. It was found that in immature cultures (DIV 11), immobilization for 1 or 2 hours did not affect the parameters measured. By contrast, at DIV 20, ischaemic conditions for 1 hour caused a pronounced decrease in the activities of glutamic acid decarboxylase and choline acetyltransferase. A significant decrease in these neuron-specific enzyme activities was found at post-ischaemic days 1-14, indicating immediate and irreversible neuronal damage. The activity of the astrocyte-specific enzyme, glutamine synthetase, was significantly increased at 4 days post-treatment; equal to control values at 6 days; and significantly decreased at 14 days after the ischaemic insult. Immobilization of DIV 20 cultures for 2 hours caused a drastic reduction in all the parameters measured at post-ischaemic day 6. Generally, the ischaemic conditions appeared to be more detrimental to neurons than to astrocytes, and GABAergic neurons were more affected than cholinergic neurons.
Keywords
aggregating brain cell cultures, ischaemia, neurotoxicity
Pubmed
Web of science
Create date
24/01/2008 13:11
Last modification date
20/08/2019 12:26
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