Antigen recognition by H-2-restricted cytolytic T lymphocytes: inhibition of cytolysis by anti-CD8 monoclonal antibodies depends upon both concentration and primary sequence of peptide antigen

Details

Serval ID
serval:BIB_0526EF8305C8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Antigen recognition by H-2-restricted cytolytic T lymphocytes: inhibition of cytolysis by anti-CD8 monoclonal antibodies depends upon both concentration and primary sequence of peptide antigen
Journal
European Journal of Immunology
Author(s)
Maryanski  J. L., Pala  P., Cerottini  J. C., MacDonald  H. R.
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
11/1988
Volume
18
Number
11
Pages
1863-6
Notes
Journal Article --- Old month value: Nov
Abstract
While it is generally agreed that the specificity of the interaction between cytolytic T lymphocytes (CTL) and their target cells is controlled mainly by antigen-specific T cell receptors (TcR), the molecular role of cell surface CD8 molecules in this interaction is less well understood. In the present study we have reinvestigated the apparent contribution of CD8 molecules to the overall avidity of interaction between CTL and their targets by using a recently developed system of major histocompatibility complex (MHC) class I-restricted CTL clones that recognize defined peptide antigens. We demonstrate that under conditions where the density of MHC, TcR and accessory molecules remains constant, the susceptibility of CD8+ CTL to inhibition of cytolysis by anti-CD8 antibodies is highly dependent upon the concentration and primary structure of the peptide antigen. Although the precise role of the CD8 molecule remains unknown, our results are compatible with models that suggest its contribution to the overall avidity of the CTL-target cell interaction particularly in cases where the affinity between the TcR and antigen-MHC is low.
Keywords
Animals Antibodies, Monoclonal Antigen-Antibody Reactions Antigen-Presenting Cells/immunology Antigens, CD8 Antigens, Differentiation/immunology Antigens, Differentiation, T-Lymphocyte/*immunology Antigens, Ly/*immunology Clone Cells Dose-Response Relationship, Immunologic H-2 Antigens/*physiology Lymphocyte Function-Associated Antigen-1 Mice Receptors, Antigen, T-Cell/*physiology T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Create date
28/01/2008 11:14
Last modification date
20/08/2019 12:26
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