Positive impact of DNA contamination minimization procedures taken within the laboratory.

Details

Ressource 1Download: 30469016_pp_cover.pdf (636.02 [Ko])
State: Public
Version: Author's accepted manuscript
License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_0489963A1FB3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Positive impact of DNA contamination minimization procedures taken within the laboratory.
Journal
Forensic science international. Genetics
Author(s)
Basset P., Castella V.
ISSN
1878-0326 (Electronic)
ISSN-L
1872-4973
Publication state
Published
Issued date
01/2019
Peer-reviewed
Oui
Volume
38
Pages
232-235
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
DNA contamination incidents are one of the most frequent sources of error in forensic genetics and can have serious consequences. It is therefore essential to take measures to prevent these events and to monitor the real impact of contamination minimization procedures. In this study, we review and compare the number of contamination events detected on trace samples analyzed by the Forensic Genetic Unit (FGU) of the University Center of Legal Medicine in Switzerland before and after the implementation of new contamination minimization procedures. Interestingly, the number of contamination events by laboratory staff was significantly reduced by more than 70% after the implementation of the procedures. However, no significant change was observed for contamination events by police collaborators. This difference is likely to be explained by the differential impact of procedures taken in the laboratory and on crime scene. It suggests that the reduction observed for laboratory contamination incidents is due to the new procedures taken. In conclusion, our study highlights that taking appropriate measures is efficient and can reduce the number of contamination incidents. However, it is important that such contamination minimization procedures be implemented all along the chain of analysis of a stain (i.e. from crime scene to the laboratory).
Keywords
DNA Contamination, Humans, Laboratories, Quality Control, Specimen Handling/standards, Switzerland, DNA contamination, Forensic DNA analysis, Recommendations, Transfer
Pubmed
Web of science
Create date
07/12/2018 10:03
Last modification date
20/08/2019 12:26
Usage data