Effects of stem cell factor and other bone marrow-derived growth factors on the expression of adhesion molecules and proliferation of human neuroblastoma cells

Details

Serval ID
serval:BIB_047B5044F5D5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effects of stem cell factor and other bone marrow-derived growth factors on the expression of adhesion molecules and proliferation of human neuroblastoma cells
Journal
European Journal of Cancer
Author(s)
Beck  D., Gross  N., Beretta Brognara  C.
ISSN
0959-8049
Publication state
Published
Issued date
1995
Peer-reviewed
Oui
Volume
31A
Number
4
Pages
467-70
Notes
Journal Article
Research Support, Non-U.S. Gov't
Abstract
Metastasis in children with neuroblastoma (NB) is a poor prognostic factor despite intensive therapy. In the near future, stem cell factor (SCF) is likely to be used clinically to accelerate bone marrow (BM) recovery after high-dose chemotherapy in patients with advanced NB. The high frequency of BM metastases in NB could be secondary to BM-derived human growth factors (HGF) modulating the adhesion, secondary growth (or both) of circulating metastatic NB cells. To test this hypothesis, we studied the in vitro effects on NB cell lines grown in chemically defined medium of SCF, interleukin (IL)-1 beta, IL-3, IL-6, basic fibroblast growth factor (bFGF), transforming growth factor-beta (TGF-beta) used alone or in combination. The antigenic expression of NB-associated cell adhesion molecules (CAM) HLA class 1, intercellular CAM-1, neural-CAM and CD44 were assayed by monoclonal antibodies and flow cytometry, and DNA synthesis by 3H-thymidine uptake. The expression of CAM was not modulated by SCF or other HGFs. An increase in thymidine uptake was induced by bFGF alone in IMR-32 cells, while SCF and other HGFs had no notable effect. Our results indicate that SCF and other BM-derived HGFs are unlikely to have a generalised effect on the expression of adhesion molecules by NB cells or proliferation. The clinical administration of recombinant human SCF to children with NB should be safe.
Keywords
Antigens, CD44/metabolism Cell Adhesion Molecules/*metabolism Cell Division/drug effects Fibroblast Growth Factor 2/pharmacology Flow Cytometry Growth Substances/*pharmacology Histocompatibility Antigens Class I/metabolism Humans Interleukins/pharmacology Neuroblastoma/*metabolism/pathology Recombinant Proteins/pharmacology Stem Cell Factor/pharmacology Transforming Growth Factor beta/pharmacology Tumor Cells, Cultured
Pubmed
Web of science
Create date
20/01/2008 15:55
Last modification date
20/08/2019 12:26
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