Expression of SCG10 and stathmin proteins in the rat olfactory system during development and axonal regeneration.

Details

Serval ID
serval:BIB_042DDF9B7BAA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Expression of SCG10 and stathmin proteins in the rat olfactory system during development and axonal regeneration.
Journal
Journal of Comparative Neurology
Author(s)
Pellier-Monnin V., Astic L., Bichet S., Riederer B.M., Grenningloh G.
ISSN
0021-9967 (Print)
ISSN-L
0021-9967
Publication state
Published
Issued date
2001
Volume
433
Number
2
Pages
239-254
Language
english
Abstract
The membrane-associated protein SCG10 is expressed specifically by neuronal cells. Recent experiments have suggested that it promotes neurite outgrowth by increasing microtubule dynamics in growth cones. SCG10 is related to the ubiquitous but neuron-enriched cytosolic protein stathmin. To better understand the role played by SCG10 and stathmin in vivo, we have analyzed the expression and localization of these proteins in both the olfactory epithelium and the olfactory bulb in developing and adult rats, as well as in adult bulbectomized rats. The olfactory epithelium is exceptional in that olfactory receptor neurons constantly regenerate and reinnervate the olfactory bulb throughout animal life-span. SCG10 and stathmin expression in the olfactory receptor neurons was found to be regulated during embryonic and postnatal development and to correlate with neuronal maturation. Whereas SCG10 expression was restricted to immature olfactory receptor neurons (GAP-43-positive, olfactory marker protein-negative), stathmin was also expressed by the basal cells. In the olfactory bulb of postnatal and adult rats, a moderate to strong SCG10 immunoreactivity was present in the olfactory nerve layer, whereas no labeling was detected in the glomerular layer. Olfactory glomeruli also showed no apparent immunoreactivity for several cytoskeletal proteins such as tubulin and microtubule-associated proteins. In unilaterally bulbectomized rats, SCG10 and stathmin were seen to be up-regulated in the regenerating olfactory epithelium at postsurgery stages corresponding to olfactory axon regeneration. Our data strongly suggest that, in vivo, both SCG10 and stathmin may play a role in axonal outgrowth during ontogenesis as well as during axonal regeneration.
Keywords
Aging/metabolism, Animals, Animals, Newborn/growth & development, Animals, Newborn/metabolism, Axons/physiology, Carrier Proteins, Cytoskeletal Proteins/metabolism, Embryo, Mammalian/physiology, Embryonic and Fetal Development, GAP-43 Protein/metabolism, Membrane Proteins, Microtubule Proteins, Nerve Growth Factors/metabolism, Nerve Regeneration/physiology, Olfactory Bulb/embryology, Olfactory Bulb/metabolism, Olfactory Mucosa/metabolism, Olfactory Pathways/physiology, Phosphoproteins/metabolism, Rats/embryology, Rats/metabolism, Rats, Wistar, Stathmin, Up-Regulation
Pubmed
Web of science
Create date
24/01/2008 14:35
Last modification date
20/08/2019 12:26
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