Prevention of murine cryoglobulinemia and associated pathology by monoclonal anti-idiotypic antibody
Details
Serval ID
serval:BIB_03B6CA53EEB9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Prevention of murine cryoglobulinemia and associated pathology by monoclonal anti-idiotypic antibody
Journal
Journal of Immunology
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
10/1989
Volume
143
Number
8
Pages
2508-13
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 15
Research Support, Non-U.S. Gov't --- Old month value: Oct 15
Abstract
A murine IgG3 mAb, clone 6-19, derived from non-manipulated autoimmune MRL/MpJ-lpr/lpr mice is a rheumatoid factor specific for IgG2a and is able to generate cryoglobulins via nonspecific IgG3 Fc-Fc interaction. Intraperitoneal passive transfer of ascites containing the 6-19 mAb into BALB/c mice induces, within 18 h, remarkable pathology characterized by skin vasculitis and acute glomerulonephritis associated with cryoglobulinemia. In order to evaluate the possibility of modulating the development of tissue lesions by an anti-Id antibody, we have raised an IgG2b anti-Id mAb specific to the 6-19 mAb. The cryoprecipitation of 6-19 mAb was completely inhibited in the presence of excess amounts of anti-Id mAb in vitro. In vivo, pretreatment of BALB/c mice with anti-6-19 anti-Id mAb inhibited development of skin vasculitis and glomerulonephritis induced by the 6-19 mAb. The cryoglobulin formation was markedly diminished due to enhanced elimination of the 6-19 mAb from the circulation. In contrast, pretreatment with an IgM anti-IgG3 rheumatoid factor mAb neither protected nor aggravated the development of tissue lesions. These results suggest possible implications in the anti-Id treatment of similar vascular diseases in man.
Keywords
Adjuvants, Immunologic/physiology/therapeutic use
Animals
Antibodies, Anti-Idiotypic/biosynthesis/physiology/*therapeutic use
Antibodies, Monoclonal/biosynthesis/physiology/*therapeutic use
Cryoglobulinemia/immunology/pathology/*prevention & control
Cryoglobulins/metabolism
Glomerulonephritis/pathology
Immunoglobulin G/physiology
Immunoglobulin Idiotypes/*immunology
Mice
Mice, Inbred BALB C
Precipitin Tests
Rheumatoid Factor/physiology
Skin/pathology
Pubmed
Web of science
Create date
25/01/2008 16:20
Last modification date
20/08/2019 13:25