Prevention of murine cryoglobulinemia and associated pathology by monoclonal anti-idiotypic antibody

Details

Serval ID
serval:BIB_03B6CA53EEB9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Prevention of murine cryoglobulinemia and associated pathology by monoclonal anti-idiotypic antibody
Journal
Journal of Immunology
Author(s)
Spertini  F., Donati  Y., Welle  I., Izui  S., Lambert  P. H.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
10/1989
Volume
143
Number
8
Pages
2508-13
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 15
Abstract
A murine IgG3 mAb, clone 6-19, derived from non-manipulated autoimmune MRL/MpJ-lpr/lpr mice is a rheumatoid factor specific for IgG2a and is able to generate cryoglobulins via nonspecific IgG3 Fc-Fc interaction. Intraperitoneal passive transfer of ascites containing the 6-19 mAb into BALB/c mice induces, within 18 h, remarkable pathology characterized by skin vasculitis and acute glomerulonephritis associated with cryoglobulinemia. In order to evaluate the possibility of modulating the development of tissue lesions by an anti-Id antibody, we have raised an IgG2b anti-Id mAb specific to the 6-19 mAb. The cryoprecipitation of 6-19 mAb was completely inhibited in the presence of excess amounts of anti-Id mAb in vitro. In vivo, pretreatment of BALB/c mice with anti-6-19 anti-Id mAb inhibited development of skin vasculitis and glomerulonephritis induced by the 6-19 mAb. The cryoglobulin formation was markedly diminished due to enhanced elimination of the 6-19 mAb from the circulation. In contrast, pretreatment with an IgM anti-IgG3 rheumatoid factor mAb neither protected nor aggravated the development of tissue lesions. These results suggest possible implications in the anti-Id treatment of similar vascular diseases in man.
Keywords
Adjuvants, Immunologic/physiology/therapeutic use Animals Antibodies, Anti-Idiotypic/biosynthesis/physiology/*therapeutic use Antibodies, Monoclonal/biosynthesis/physiology/*therapeutic use Cryoglobulinemia/immunology/pathology/*prevention & control Cryoglobulins/metabolism Glomerulonephritis/pathology Immunoglobulin G/physiology Immunoglobulin Idiotypes/*immunology Mice Mice, Inbred BALB C Precipitin Tests Rheumatoid Factor/physiology Skin/pathology
Pubmed
Web of science
Create date
25/01/2008 16:20
Last modification date
20/08/2019 13:25
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