Connexin-36 contributes to control function of insulin-producing cells.

Details

Serval ID
serval:BIB_0359C7FDA2B8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Connexin-36 contributes to control function of insulin-producing cells.
Journal
Journal of Biological Chemistry
Author(s)
Le Gurun S., Martin D., Formenton A., Maechler P., Caille D., Waeber G., Meda P., Haefliger J.A.
ISSN
0021-9258
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
278
Number
39
Pages
37690-37697
Language
english
Abstract
Connexin-36 (Cx36) is a gap junction protein expressed by the insulin-producing beta-cells. We investigated the contribution of this protein in normal beta-cell function by using a viral gene transfer approach to alter Cx36 content in the insulin-producing line of INS-1E cells and rat pancreatic islets. Transcripts for Cx43, Cx45, and Cx36 were detected by reverse transcriptase-PCR in freshly isolated pancreatic islets, whereas only a transcript for Cx36 was detected in INS-1E cells. After infection with a sense viral vector, which induced de novo Cx36 expression in the Cx-defective HeLa cells we used to control the transgene expression, Western blot, immunofluorescence, and freeze-fracture analysis showed a large increase of Cx36 within INS-1E cell membranes. In contrast, after infection with an antisense vector, Cx36 content was decreased by 80%. Glucose-induced insulin release and insulin content were decreased, whether infected INS-1E cells expressed Cx36 levels that were largely higher or lower than those observed in wild-type control cells. In both cases, basal insulin secretion was unaffected. Comparable observations on basal secretion and insulin content were made in freshly isolated rat pancreatic islets. The data indicate that large changes in Cx36 alter insulin content and, at least in INS-1E cells, also affect glucose-induced insulin release.
Keywords
Adenoviridae/genetics, Amino Acid Sequence, Animals, Calcium/metabolism, Cell Line, Connexins/physiology, Insulin/analysis, Insulin/secretion, Islets of Langerhans/physiology, Molecular Sequence Data, Rats
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 14:48
Last modification date
20/08/2019 13:25
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