Tumour-reactive T cell subsets in the microenvironment of ovarian cancer.

Details

Serval ID
serval:BIB_031BC21D26AB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Tumour-reactive T cell subsets in the microenvironment of ovarian cancer.
Journal
British journal of cancer
Author(s)
Westergaard MCW, Andersen R., Chong C., Kjeldsen J.W., Pedersen M., Friese C., Hasselager T., Lajer H., Coukos G., Bassani-Sternberg M., Donia M., Svane I.M.
ISSN
1532-1827 (Electronic)
ISSN-L
0007-0920
Publication state
Published
Issued date
02/2019
Peer-reviewed
Oui
Volume
120
Number
4
Pages
424-434
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Solid malignancies are frequently infiltrated with T cells. The success of adoptive cell transfer (ACT) with expanded tumour-infiltrating lymphocytes (TILs) in melanoma warrants its testing in other cancer types. In this preclinical study, we investigated whether clinical-grade TILs could be manufactured from ovarian cancer (OC) tumour specimens.
Thirty-four tumour specimens were obtained from 33 individual patients with OC. TILs were analysed for phenotype, antigen specificity and functionality.
Minimally expanded TILs (Young TILs) were successfully established from all patients. Young TILs contained a high frequency of CD3 <sup>+</sup> cells with a variable CD4/CD8 ratio. TILs could be expanded to clinical numbers. Importantly, recognition of autologous tumour cells was demonstrated in TILs in >50% of the patients. We confirmed with mass spectrometry the presentation of multiple tumour antigens, including peptides derived from the cancer-testis antigen GAGE, which could be recognised by antigen-specific TILs. Antigen-specific TILs could be isolated and further expanded in vitro.
These findings support the hypothesis that patients with OC can benefit from ACT with TILs and led to the initiation of a pilot clinical trial at our institution .
clinicaltrials.gov: NCT02482090.
Pubmed
Web of science
Create date
11/03/2019 17:26
Last modification date
20/08/2019 12:25
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