Inducible Hsp70 as target of anticancer immunotherapy: Identification of HLA-A*0201-restricted epitopes.

Details

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State: Public
Version: Final published version
Serval ID
serval:BIB_02B4C064F3C8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Inducible Hsp70 as target of anticancer immunotherapy: Identification of HLA-A*0201-restricted epitopes.
Journal
International Journal of Cancer
Author(s)
Faure O., Graff-Dubois S., Bretaudeau L., Derré L., Gross D.A., Alves P.M., Cornet S., Duffour M.T., Chouaib S., Miconnet I., Grégoire M., Jotereau F., Lemonnier F.A., Abastado J.P., Kosmatopoulos K.
ISSN
0020-7136 (Print)
ISSN-L
0020-7136
Publication state
Published
Issued date
2004
Volume
108
Number
6
Pages
863-870
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The design of a broad application tumor vaccine requires the identification of tumor antigens expressed in a majority of tumors of various origins. We questioned whether the major stress-inducible heat shock protein Hsp70 (also known as Hsp72), a protein frequently overexpressed in human tumors of various histological origins, but not in most physiological normal tissues, constitutes a tumor antigen. We selected the p391 and p393 peptides from the sequence of the human inducible Hsp70 that had a high affinity for HLA-A*0201. These peptides were able to trigger a CTL response in vivo in HLA-A*0201-transgenic HHD mice and in vitro in HLA-A*0201+ healthy donors. p391- and p393-specific human and murine CTL recognized human tumor cells overexpressing Hsp70 in a HLA-A*0201-restricted manner. Tetramer analysis of TILs showed that these Hsp70 epitopes are targets of an immune response in many HLA-A*0201+ breast cancer patients. Hsp70 is a tumor antigen and the Hsp70-derived peptides p391 and p393 could be used to raise a cytotoxic response against tumors of various origins.
Keywords
Animals, Antigens, Neoplasm/chemistry, Blotting, Western, CD8-Positive T-Lymphocytes/metabolism, COS Cells, Cell Line, Tumor, Dose-Response Relationship, Drug, Epitopes/chemistry, HLA Antigens/chemistry, HLA-A Antigens/chemistry, HLA-A2 Antigen, HSP70 Heat-Shock Proteins/metabolism, Humans, Immunotherapy/methods, Interferon-gamma/metabolism, Mice, Mice, Transgenic, Peptides/chemistry, Plasmids/metabolism, T-Lymphocytes, Cytotoxic/metabolism, Tumor Necrosis Factor-alpha/metabolism
Pubmed
Web of science
Create date
25/01/2008 16:08
Last modification date
20/08/2019 13:24
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