A glycine-arginine domain in control of the human MRE11 DNA repair protein.

Details

Serval ID
serval:BIB_02AB3CC30E0D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A glycine-arginine domain in control of the human MRE11 DNA repair protein.
Journal
Molecular and Cellular Biology
Author(s)
Déry U., Coulombe Y., Rodrigue A., Stasiak A., Richard S., Masson J.Y.
ISSN
1098-5549[electronic]
Publication state
Published
Issued date
05/2008
Peer-reviewed
Oui
Volume
28
Number
9
Pages
3058-3069
Language
english
Abstract
Human MRE11 is a key enzyme in DNA double-strand break repair and genome stability. Human MRE11 bears a glycine-arginine-rich (GAR) motif that is conserved among multicellular eukaryotic species. We investigated how this motif influences MRE11 function. Human MRE11 alone or a complex of MRE11, RAD50, and NBS1 (MRN) was methylated in insect cells, suggesting that this modification is conserved during evolution. We demonstrate that PRMT1 interacts with MRE11 but not with the MRN complex, suggesting that MRE11 arginine methylation occurs prior to the binding of NBS1 and RAD50. Moreover, the first six methylated arginines are essential for the regulation of MRE11 DNA binding and nuclease activity. The inhibition of arginine methylation leads to a reduction in MRE11 and RAD51 focus formation on a unique double-strand break in vivo. Furthermore, the MRE11-methylated GAR domain is sufficient for its targeting to DNA damage foci and colocalization with gamma-H2AX. These studies highlight an important role for the GAR domain in regulating MRE11 function at the biochemical and cellular levels during DNA double-strand break repair.
Keywords
Amino Acid Motifs, Animals, Arginine/metabolism, Cell Cycle Proteins/metabolism, Cell Line, DNA Breaks, Double-Stranded, DNA Repair, DNA Repair Enzymes/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Glycine/metabolism, Histones/metabolism, Humans, Methylation, Nuclear Proteins/metabolism, Protein Binding, Protein-Arginine N-Methyltransferase/metabolism, Rad51 Recombinase/metabolism, Recombinant Proteins/metabolism, Repressor Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
26/11/2008 15:19
Last modification date
20/08/2019 12:24
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