Influence of cytoskeleton organization on recombinant protein expression by CHO cells.

Details

Serval ID
serval:BIB_02A3B3842F19
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Influence of cytoskeleton organization on recombinant protein expression by CHO cells.
Journal
Biotechnology and bioengineering
Author(s)
Pourcel L., Buron F., Arib G., Le Fourn V., Regamey A., Bodenmann I., Girod P.A., Mermod N.
ISSN
1097-0290 (Electronic)
ISSN-L
0006-3592
Publication state
Published
Issued date
04/2020
Peer-reviewed
Oui
Volume
117
Number
4
Pages
1117-1126
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
In this study, we assessed the importance of cytoskeleton organization in the mammalian cells used to produce therapeutic proteins. Two cytoskeletal genes, Actin alpha cardiac muscle 1 (ACTC1) and a guanosine triphosphate GTPase-activating protein (TAGAP), were found to be upregulated in highly productive therapeutic protein-expressing Chinese hamster ovary (CHO) cells selected by the deprivation of vitamin B5. We report here that the overexpression of the ACTC1 protein was able to improve significantly recombinant therapeutic production, as well as to decrease the levels of toxic lactate metabolic by-products. ACTC1 overexpression was accompanied by altered as well as decreased polymerized actin, which was associated with high protein production by CHO cell cultured in suspension. We suggest that the depolymerization of actin and the possible modulation of integrin signaling, as well as changes in basal metabolism, may be driving the increase of protein secretion by CHO cells.
Keywords
CHO cells, actin polymerization, cytoskeleton regulation, recombinant protein production
Pubmed
Web of science
Open Access
Yes
Create date
23/01/2020 14:15
Last modification date
13/06/2020 6:20
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