Regeneration patterns influence hindlimb automutilation after sciatic nerve repair using stem cells in rats.

Details

Serval ID
serval:BIB_029526882C4D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Regeneration patterns influence hindlimb automutilation after sciatic nerve repair using stem cells in rats.
Journal
Neuroscience Letters
Author(s)
Haselbach D., Raffoul W., Larcher L., Tremp M., Kalbermatten D.F., di Summa P.G.
ISSN
1872-7972 (Electronic)
ISSN-L
0304-3940
Publication state
Published
Issued date
2016
Volume
634
Pages
153-159
Language
english
Notes
Publication types: ARTICLE
Publication Status: aheadofprint
Abstract
INTRODUCTION: Hindlimb autophagy is common after rat sciatic total axotomy and is considered as a sign of neuropathic pain. We applied adult stem cells in a fibrin conduit in a total sciatic axotomy model to improve nerve regeneration, investigating whether a correlation could be detected between stem cells effects on regeneration and limb autophagy.
MATERIAL AND METHODS: After sciatic nerve section, a 1-cm sciatic gap was crossed using fibrin conduits. Experimental groups included empty fibrin conduits, fibrin conduits seeded with primary Schwann cells, and fibrin conduits seeded with Schwann cell-like differentiated mesenchymal or adipose-derived stem cells (dMSCs and dASCs). Controls were represented by autografts and by sham rats (tot n=34). At 16 weeks post-implantation, regeneration pattern was analysed on histological sections and related to eventual autophagy. Hindlimbs were evaluated and scored according to autophagy Wall's scale and X-Rays radiological evaluation.
RESULTS: All regenerative cell lines significantly improved myelination at the mid conduit level, compared to the empty tubes. However, dMSC could not significantly improve myelination at the distal stump, showing a more chaotic regeneration compared to both other cells groups and controls. Autophagy was correlated to this regeneration patterns, with higher autophagy scores in the empty and dMSC group.
CONCLUSION: Hindlimb autophagy can be used as index of neuropathic pain due to nerve lesion or on-going immature regeneration. dMSC group was characterized by a less targeted regeneration comparing to dASC and primary Schwann cells, which confirmed their effectiveness in regeneration and potential in future clinical applications.
Pubmed
Create date
23/10/2016 16:44
Last modification date
02/06/2020 6:26
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