Patterns of oxygen consumption during establishment of cephalocaudal polarity in the early chick embryo.

Details

Serval ID
serval:BIB_027DDC13C4A1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Patterns of oxygen consumption during establishment of cephalocaudal polarity in the early chick embryo.
Journal
Journal of Experimental Zoology. Supplement
Author(s)
Raddatz E., Eyal-Giladi H., Kucera P.
ISSN
1059-8324 (Print)
ISSN-L
1059-8324
Publication state
Published
Issued date
1987
Volume
1
Pages
213-218
Language
english
Abstract
Oxygen uptake was studied during the establishment of cephalocaudal polarity in the very early chick embryo, i.e., 10 hr before (stage VI) and at laying (stage X). Oxygen fluxes in minute regions of the intact blastoderms were measured in vitro by scanning microspectrophotometry in the presence or absence of glucose. The oxygen consumption of the whole blastoderm remained constant (6 nmol O2 X hr-1) throughout the period studied, although the number of cells increased more than twofold. The regional oxygen fluxes varied from 0.41 to 1.13 nmol O2 X hr-1 X mm-2 at stage VI and from 0.42 to 0.70 nmol O2 X hr-1 X mm-2 at stage X. At stage VI, the oxygen flux in the center of the blastoderm was significantly higher than that in its periphery. This pattern remained evident when the values were corrected for cell number or for cytoplasmic volume. At stage X, there was a tendency for the oxygen fluxes to decrease from the posterior to the anterior regions of the area pellucida. Thus the pattern of oxidative metabolism in the late uterine embryos seems to change from radial to bilateral. This change of symmetry probably reflects the process of formation of the embryonic axis. In addition, the fact that the oxygen uptake was similar in the presence or absence of glucose suggests that early chick embryos metabolize essentially intracellular stores.
Keywords
Analysis of Variance, Animals, Blastoderm/metabolism, Chick Embryo/growth & development, Chick Embryo/metabolism, Glucose/metabolism, Glycogen/analysis, Oxygen Consumption, Proteins/analysis
Pubmed
Create date
24/01/2008 13:19
Last modification date
20/08/2019 12:24
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