β-Catenin Expression and Activation in Conjunctival Melanoma.

Details

Ressource 1Download: riggi_dermato.pdf (2242.74 [Ko])
State: Public
Version: Final published version
License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_026BB5F40479
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
β-Catenin Expression and Activation in Conjunctival Melanoma.
Journal
Dermatopathology
Author(s)
Larivé E., Nicolas M., Kaya G., Riggi N., Moulin A.P.
ISSN
2296-3529 (Print)
ISSN-L
2296-3529
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Volume
6
Number
2
Pages
50-62
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
To assess the role of the canonical Wnt pathway via activation of β-catenin in tumor progression of conjunctival melanoma.
β-Catenin localization was assessed by immunohistochemistry in 43 conjunctival nevi, 48 primary acquired melanoses (PAM; conjunctival melanocytic intraepithelial neoplasia), and 44 conjunctival melanomas. Activation of the canonical or the noncanonical Wnt pathway was tested in vitro in 4 conjunctival melanoma cell lines with stimulation of either Wnt5a or Wnt3a. Wound healing assays were performed with Wnt5a.
Nuclear β-catenin expression was found in 16% of the nevi, in 15% of the melanomas, and in 4% of the PAM. Membranous β-catenin expression was identified in all the nevi and PAM and in 97.7% of the melanomas. In vitro, Wnt5a stimulation in the 4 conjunctival melanomas and in 1 skin melanoma cell line did not induce nuclear translocation of β-catenin, nor did it increase cell motility in the wound healing assays. Wnt3a stimulation did not induce nuclear localization of β-catenin in any of the cell lines tested.
In conjunctival melanoma, nuclear localization and activation of β-catenin appear to be limited, suggesting that inhibition of ARF6, responsible for β-catenin activation, in subsets of skin melanoma may not represent a treatment option for this tumor. In vitro, Wnt3a or Wnt5a did not induce nuclear β-catenin localization.
Keywords
Conjunctiva, Eye, Melanogenesis, Melanoma
Pubmed
Web of science
Open Access
Yes
Create date
07/08/2019 9:20
Last modification date
15/11/2019 6:18
Usage data