Global Isotope Metabolomics Reveals Adaptive Strategies for Nitrogen Assimilation.

Details

Serval ID
serval:BIB_023B760514DF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Global Isotope Metabolomics Reveals Adaptive Strategies for Nitrogen Assimilation.
Journal
ACS chemical biology
Author(s)
Kurczy M.E., Forsberg E.M., Thorgersen M.P., Poole F.L., Benton H.P., Ivanisevic J., Tran M.L., Wall J.D., Elias D.A., Adams M.W., Siuzdak G.
ISSN
1554-8937 (Electronic)
ISSN-L
1554-8929
Publication state
Published
Issued date
17/06/2016
Peer-reviewed
Oui
Volume
11
Number
6
Pages
1677-1685
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Nitrogen cycling is a microbial metabolic process essential for global ecological/agricultural balance. To investigate the link between the well-established ammonium and the alternative nitrate assimilation metabolic pathways, global isotope metabolomics was employed to examine three nitrate reducing bacteria using (15)NO3 as a nitrogen source. In contrast to a control (Pseudomonas stutzeri RCH2), the results show that two of the isolates from Oak Ridge, Tennessee (Pseudomonas N2A2 and N2E2) utilize nitrate and ammonia for assimilation concurrently with differential labeling observed across multiple classes of metabolites including amino acids and nucleotides. The data reveal that the N2A2 and N2E2 strains conserve nitrogen-containing metabolites, indicating that the nitrate assimilation pathway is a conservation mechanism for the assimilation of nitrogen. Co-utilization of nitrate and ammonia is likely an adaption to manage higher levels of nitrite since the denitrification pathways utilized by the N2A2 and N2E2 strains from the Oak Ridge site are predisposed to the accumulation of the toxic nitrite. The use of global isotope metabolomics allowed for this adaptive strategy to be investigated, which would otherwise not have been possible to decipher.

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Create date
14/04/2016 16:28
Last modification date
20/08/2019 12:24
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