Combined Action of Piperitenone Epoxide and Antibiotics Against Clinical Isolates of Staphylococcus aureus and Escherichia coli.

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State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_01C9A4387291
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Combined Action of Piperitenone Epoxide and Antibiotics Against Clinical Isolates of Staphylococcus aureus and Escherichia coli.
Journal
Frontiers in microbiology
Author(s)
Alexopoulos A., Kimbaris A.C., Plessas S., Mantzourani I., Voidarou C., Pagonopoulou O., Tsigalou C., Fournomiti M., Bontsidis C., Stavropoulou E., Papaemmanouil V., Bezirtzoglou E.
ISSN
1664-302X (Print)
ISSN-L
1664-302X
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Volume
10
Pages
2607
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
The aim of this study was to determine the antimicrobial efficiency of piperitenone epoxide (PEO) - a principal component of various aromatic plants' essential oil - in combination with various antibiotics against 28 strains of Staphylococcus aureus and 10 strains of Escherichia coli isolated from clinical samples. Mentha spicata's essential oil, initially collected by hydrodistillation, was then subjected to flush column chromatography affording PEO of high purity. Minimum inhibitory concentrations of PEO alone and in combination with various concentrations of antibiotics were assessed using the microdilution method. The combined action was estimated calculating the fractional inhibitory concentration (FIC) index from checkerboard assays. Our results showed that the average minimum inhibitory concentration (mg/l) of PEO alone against E. coli was 512 ± 364.7 μg/ml, which was significantly higher than 172.8 ± 180.7 μg/ml observed for S. aureus. From checkerboard assays, FIC values below the 0.5 index, indicating synergy, were observed for 59% of the drugs tested. Twelve percent of FIC index values were between 0.5 and 1, indicating additive effects, while 21% were indifferent. According to our results, PEO could be a promising antimicrobial compound when combined with specific antibiotics and deserves further study.
Keywords
Escherichia coli, Staphylococcus aureus, antibiotics, fractional inhibitory concentration, fractional inhibitory concentration index, minimum inhibitory concentration, piperitenone epoxide
Pubmed
Web of science
Open Access
Yes
Create date
15/12/2019 17:52
Last modification date
08/08/2024 6:29
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