Immunogold Detection of L-glutamate and D-serine in Small Synaptic-Like Microvesicles in Adult Hippocampal Astrocytes.

Details

Ressource 1Download: serval:BIB_019C2C44FB47.P001 (576.38 [Ko])
State: Public
Version: author
License: Not specified
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
Serval ID
serval:BIB_019C2C44FB47
Type
Article: article from journal or magazin.
Collection
Publications
Title
Immunogold Detection of L-glutamate and D-serine in Small Synaptic-Like Microvesicles in Adult Hippocampal Astrocytes.
Journal
Cerebral Cortex
Author(s)
Bergersen L.H., Morland C., Ormel L., Rinholm J.E., Larsson M., Wold J.F., Røe A.T., Stranna A., Santello M., Bouvier D., Ottersen O.P., Volterra A., Gundersen V.
ISSN
1460-2199 (Electronic)
ISSN-L
1047-3211
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
22
Number
7
Pages
1690-1697
Language
english
Abstract
Glutamate and the N-methyl-D-aspartate receptor ligand D-serine are putative gliotransmitters. Here, we show by immunogold cytochemistry of the adult hippocampus that glutamate and D-serine accumulate in synaptic-like microvesicles (SLMVs) in the perisynaptic processes of astrocytes. The estimated concentration of fixed glutamate in the astrocytic SLMVs is comparable to that in synaptic vesicles of excitatory nerve terminals (∼45 and ∼55 mM, respectively), whereas the D-serine level is about 6 mM. The vesicles are organized in small spaced clusters located near the astrocytic plasma membrane. Endoplasmic reticulum is regularly found in close vicinity to SLMVs, suggesting that astrocytes contain functional nanodomains, where a local Ca(2+) increase can trigger release of glutamate and/or D-serine.
Pubmed
Web of science
Open Access
Yes
Create date
23/07/2012 10:38
Last modification date
24/09/2019 9:01
Usage data