Steatosis is not sufficient to cause an impaired regenerative response after partial hepatectomy in rats.

Details

Serval ID
serval:BIB_01970932B40A
Type
Article: article from journal or magazin.
Collection
Publications
Title
Steatosis is not sufficient to cause an impaired regenerative response after partial hepatectomy in rats.
Journal
Journal of Hepatology
Author(s)
Picard C., Lambotte L., Starkel P., Sempoux C., Saliez A., Van den Berge V., Horsmans Y.
ISSN
0168-8278 (Print)
ISSN-L
0168-8278
Publication state
Published
Issued date
2002
Peer-reviewed
Oui
Volume
36
Number
5
Pages
645-652
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
BACKGROUND/AIMS: Fatty liver is known to be associated with increased mortality and morbidity after liver resection. The ability of fatty liver to regenerate after two-thirds partial hepatectomy was studied in three different models of steatosis in rats: obese Zucker rats, orotic acid-fed Wistar rats and Wistar rats fed a methionine-low, choline-deficient diet.
METHODS: Liver regeneration was assessed 24 h after partial hepatectomy by bromodeoxyuridine incorporation (immunohistochemistry), proliferating cell nuclear antigen, cyclin E and cyclin-dependent kinase 2 protein expression (Western blot analysis) and cyclin-dependent kinase 2 activity (kinase assays using histone H1 as a substrate).
RESULTS: No significant difference of proliferative response was found between orotic acid or methionine-low, choline-deficient diet-fed and control Wistar rats 24 h after partial hepatectomy. In contrast, hepatocyte proliferation in obese Zucker rats after partial hepatectomy was significantly reduced when compared with their lean controls.
CONCLUSIONS: Steatosis per se does not impair liver regeneration. The reduced liver regeneration observed in obese Zucker rats may not be due to fatty infiltration itself but to other factors such as leptin receptor dysfunction.
Keywords
Animal Feed, Animals, Blotting, Western, Bromodeoxyuridine, CDC2-CDC28 Kinases, Cholesterol/analysis, Choline, Cyclin E/analysis, Cyclin-Dependent Kinase 2, Cyclin-Dependent Kinases/analysis, Disease Models, Animal, Fatty Liver/chemically induced, Fatty Liver/pathology, Hepatectomy, Liver/chemistry, Liver/pathology, Liver Regeneration/physiology, Male, Methionine, Organ Size, Orotic Acid, Proliferating Cell Nuclear Antigen/analysis, Protein-Serine-Threonine Kinases/analysis, Rats, Rats, Wistar, Rats, Zucker, Triglycerides/analysis
Pubmed
Web of science
Create date
20/10/2016 17:30
Last modification date
03/03/2018 13:15
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