HAB1-SWI3B interaction reveals a link between abscisic acid signaling and putative SWI/SNF chromatin-remodeling complexes in Arabidopsis.

Details

Serval ID
serval:BIB_0181F3DC37C2
Type
Article: article from journal or magazin.
Collection
Publications
Title
HAB1-SWI3B interaction reveals a link between abscisic acid signaling and putative SWI/SNF chromatin-remodeling complexes in Arabidopsis.
Journal
The Plant cell
Author(s)
Saez A., Rodrigues A., Santiago J., Rubio S., Rodriguez P.L.
ISSN
1040-4651 (Print)
ISSN-L
1040-4651
Publication state
Published
Issued date
11/2008
Peer-reviewed
Oui
Volume
20
Number
11
Pages
2972-2988
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Abscisic acid (ABA) has an important role for plant growth, development, and stress adaptation. HYPERSENSITIVE TO ABA1 (HAB1) is a protein phosphatase type 2C that plays a key role as a negative regulator of ABA signaling; however, the molecular details of HAB1 action in this process are not known. A two-hybrid screen revealed that SWI3B, an Arabidopsis thaliana homolog of the yeast SWI3 subunit of SWI/SNF chromatin-remodeling complexes, is a prevalent interacting partner of HAB1. The interaction mapped to the N-terminal half of SWI3B and required an intact protein phosphatase catalytic domain. Bimolecular fluorescence complementation and coimmunoprecipitation assays confirmed the interaction of HAB1 and SWI3B in the nucleus of plant cells. swi3b mutants showed a reduced sensitivity to ABA-mediated inhibition of seed germination and growth and reduced expression of the ABA-responsive genes RAB18 and RD29B. Chromatin immunoprecipitation experiments showed that the presence of HAB1 in the vicinity of RD29B and RAB18 promoters was abolished by ABA, which suggests a direct involvement of HAB1 in the regulation of ABA-induced transcription. Additionally, our results uncover SWI3B as a novel positive regulator of ABA signaling and suggest that HAB1 modulates ABA response through the regulation of a putative SWI/SNF chromatin-remodeling complex.
Keywords
Abscisic Acid/metabolism, Arabidopsis/genetics, Arabidopsis/metabolism, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Chromatin Assembly and Disassembly, Gene Expression Regulation, Plant, Germination, Mutation, Phosphoprotein Phosphatases/genetics, Phosphoprotein Phosphatases/metabolism, Plants, Genetically Modified/genetics, Plants, Genetically Modified/metabolism, Protein Interaction Mapping, RNA, Plant/genetics, RNA-Binding Proteins/genetics, RNA-Binding Proteins/metabolism, Tobacco/genetics, Tobacco/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
10/01/2019 17:30
Last modification date
21/08/2019 5:35
Usage data